TY - JOUR
T1 - Using sludge fermentation liquid to reduce the inhibitory effect of copper oxide nanoparticles on municipal wastewater biological nutrient removal
AU - Su, Yinglong
AU - Chen, Yinguang
AU - Zheng, Xiong
AU - Wan, Rui
AU - Huang, Haining
AU - Li, Mu
AU - Wu, Lijuan
N1 - Publisher Copyright:
© 2016 Elsevier Ltd.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - The deterioration of biological nutrient removal (BNR) can occur with the release of engineering nanomaterials into wastewater treatment plants (WWTPs). Also, large amounts of waste sludge are generated in WWTPs, which can be reutilized as a useful resource. In this study, the use of sludge fermentation liquid to reduce CuO nanoparticles (NPs) toxicity to municipal wastewater BNR was reported. In the BNR system supplemented with sodium acetate, which was widely used as additional carbon source of municipal wastewater in literatures, the appearance of 2.5 mg/L CuO NPs for 5.5 h decreased the total nitrogen (TN) removal efficiency from 81.4% to 59.0%, but the TN removal was recovered to 78.7% after sodium acetate was replaced by sludge fermentation liquid. It was found that CuO NPs induced excessive generation of reactive nitrogen species (RNS), which led to the disorder of redox status, low levels of energy and reduction equivalents generations, and deterioration of denitrification. Further investigation revealed that cysteine in fermentation liquid played a vital biological role in reducing nanotoxicity by facilitating the synthesis of glutathione, which reduced excessive RNS generation, increased key proteins expression, guaranteed the metabolisms of intracellular energy and substrate smoothly, and finally recovered the BNR performance.
AB - The deterioration of biological nutrient removal (BNR) can occur with the release of engineering nanomaterials into wastewater treatment plants (WWTPs). Also, large amounts of waste sludge are generated in WWTPs, which can be reutilized as a useful resource. In this study, the use of sludge fermentation liquid to reduce CuO nanoparticles (NPs) toxicity to municipal wastewater BNR was reported. In the BNR system supplemented with sodium acetate, which was widely used as additional carbon source of municipal wastewater in literatures, the appearance of 2.5 mg/L CuO NPs for 5.5 h decreased the total nitrogen (TN) removal efficiency from 81.4% to 59.0%, but the TN removal was recovered to 78.7% after sodium acetate was replaced by sludge fermentation liquid. It was found that CuO NPs induced excessive generation of reactive nitrogen species (RNS), which led to the disorder of redox status, low levels of energy and reduction equivalents generations, and deterioration of denitrification. Further investigation revealed that cysteine in fermentation liquid played a vital biological role in reducing nanotoxicity by facilitating the synthesis of glutathione, which reduced excessive RNS generation, increased key proteins expression, guaranteed the metabolisms of intracellular energy and substrate smoothly, and finally recovered the BNR performance.
KW - Nanotoxicity
KW - Protein expression
KW - Reactive nitrogen species
KW - Redox balance
KW - Sludge fermentation liquid
UR - https://www.scopus.com/pages/publications/84964949936
U2 - 10.1016/j.watres.2016.04.066
DO - 10.1016/j.watres.2016.04.066
M3 - 文章
C2 - 27161888
AN - SCOPUS:84964949936
SN - 0043-1354
VL - 99
SP - 216
EP - 224
JO - Water Research
JF - Water Research
ER -
