Uric acid detection using quartz crystal microbalance coated with uricase immobilized on ZnO nanotetrapods

Xiaohua Wang; Yinglong Yao; Jian Zhang; Ziqiang Zhu; Jianzhong Zhu

Uric acid detection using quartz crystal microbalance coated with uricase immobilized on ZnO nanotetrapods

Xiaohua Wang^*, Yinglong Yao, Jian Zhang, Ziqiang Zhu, Jianzhong Zhu

^*Corresponding author for this work

School of Communication and Electronic Engineering

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The sensing properties of uricase immobilized on ZnO nanotetrapods for uric acid detection were studied using a quartz crystal microbalance (QCM). The ZnO nanotetrapods were synthesized by thermally evaporating high-purity zinc pellets (99.999%) at 900°C in air. The sensors were constructed by distributing the ZnO nanotetrapods onto the electrode surfaces of the QCM at room temperature. Then uricase was directly immobilized on the ZnO nanotetrapods for uric acid detection. Our results indicated that this QCM sensor shows high sensitivity (10⁶ Hz/mol/L) for uric acid detection in a liquid environment. The frequency response of the sensor showed a linear (r = 0.99872) dependence on uric acid concentration ranging from 5.0×10⁶ to 1.0×10^-3 mol L^-1 with a detection limit of 2.0×10^-6 mol L^-1. All the results demonstrated that the one-dimensional ZnO structure is a good material for biosensor application.

Original language	English
Pages (from-to)	111-121
Number of pages	11
Journal	Sensors and Materials
Volume	20
Issue number	3
State	Published - 2008

Keywords

Biosensor
Immobilization
Quartz crystal microbalance
Uric acid
Uricase
ZnO nanotetrapods

Cite this

@article{3f9edde64bb247578eaf84896fa0a4bf,

title = "Uric acid detection using quartz crystal microbalance coated with uricase immobilized on ZnO nanotetrapods",

abstract = "The sensing properties of uricase immobilized on ZnO nanotetrapods for uric acid detection were studied using a quartz crystal microbalance (QCM). The ZnO nanotetrapods were synthesized by thermally evaporating high-purity zinc pellets (99.999\%) at 900°C in air. The sensors were constructed by distributing the ZnO nanotetrapods onto the electrode surfaces of the QCM at room temperature. Then uricase was directly immobilized on the ZnO nanotetrapods for uric acid detection. Our results indicated that this QCM sensor shows high sensitivity (106 Hz/mol/L) for uric acid detection in a liquid environment. The frequency response of the sensor showed a linear (r = 0.99872) dependence on uric acid concentration ranging from 5.0×106 to 1.0×10-3 mol L-1 with a detection limit of 2.0×10-6 mol L-1. All the results demonstrated that the one-dimensional ZnO structure is a good material for biosensor application.",

keywords = "Biosensor, Immobilization, Quartz crystal microbalance, Uric acid, Uricase, ZnO nanotetrapods",

author = "Xiaohua Wang and Yinglong Yao and Jian Zhang and Ziqiang Zhu and Jianzhong Zhu",

year = "2008",

language = "英语",

volume = "20",

pages = "111--121",

journal = "Sensors and Materials",

issn = "0914-4935",

publisher = "M Y U Scientific Publishing Division",

number = "3",

}

TY - JOUR

T1 - Uric acid detection using quartz crystal microbalance coated with uricase immobilized on ZnO nanotetrapods

AU - Wang, Xiaohua

AU - Yao, Yinglong

AU - Zhang, Jian

AU - Zhu, Ziqiang

AU - Zhu, Jianzhong

PY - 2008

Y1 - 2008

N2 - The sensing properties of uricase immobilized on ZnO nanotetrapods for uric acid detection were studied using a quartz crystal microbalance (QCM). The ZnO nanotetrapods were synthesized by thermally evaporating high-purity zinc pellets (99.999%) at 900°C in air. The sensors were constructed by distributing the ZnO nanotetrapods onto the electrode surfaces of the QCM at room temperature. Then uricase was directly immobilized on the ZnO nanotetrapods for uric acid detection. Our results indicated that this QCM sensor shows high sensitivity (106 Hz/mol/L) for uric acid detection in a liquid environment. The frequency response of the sensor showed a linear (r = 0.99872) dependence on uric acid concentration ranging from 5.0×106 to 1.0×10-3 mol L-1 with a detection limit of 2.0×10-6 mol L-1. All the results demonstrated that the one-dimensional ZnO structure is a good material for biosensor application.

AB - The sensing properties of uricase immobilized on ZnO nanotetrapods for uric acid detection were studied using a quartz crystal microbalance (QCM). The ZnO nanotetrapods were synthesized by thermally evaporating high-purity zinc pellets (99.999%) at 900°C in air. The sensors were constructed by distributing the ZnO nanotetrapods onto the electrode surfaces of the QCM at room temperature. Then uricase was directly immobilized on the ZnO nanotetrapods for uric acid detection. Our results indicated that this QCM sensor shows high sensitivity (106 Hz/mol/L) for uric acid detection in a liquid environment. The frequency response of the sensor showed a linear (r = 0.99872) dependence on uric acid concentration ranging from 5.0×106 to 1.0×10-3 mol L-1 with a detection limit of 2.0×10-6 mol L-1. All the results demonstrated that the one-dimensional ZnO structure is a good material for biosensor application.

KW - Biosensor

KW - Immobilization

KW - Quartz crystal microbalance

KW - Uric acid

KW - Uricase

KW - ZnO nanotetrapods

UR - https://www.scopus.com/pages/publications/49049095285

M3 - 文章

AN - SCOPUS:49049095285

SN - 0914-4935

VL - 20

SP - 111

EP - 121

JO - Sensors and Materials

JF - Sensors and Materials

IS - 3

ER -

Uric acid detection using quartz crystal microbalance coated with uricase immobilized on ZnO nanotetrapods

Abstract

Keywords

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