Understanding mechanisms of sludge in situ reduction in anaerobic side-stream reactor coupled membrane bioreactors packed with carriers at different filling fractions

An anoxic/oxic membrane bioreactor (AO) and three pilot-scale anaerobic side stream reactors (ASSR) coupled MBRs (ASSR-MBRs), packed with 0%, 25% and 50% carriers in ASSRs, were continuously operated to study the mechanisms for sludge reduction. Four systems showed efficient COD and NH₄⁺-N removal, while packing carriers significantly enhanced nitrogen removal. 25% filling fraction (AP₂₅) achieved the highest sludge reduction efficiency of 50.5% compared to 0% (21.7%) and 50% (39.7%). Compared to ASSR-MBR, carriers enhanced the release of dissolved organic matters, and accelerated the secretion of enzyme for cell lysis and hydrolysis. In AP₂₅, the presence of carriers prompted the formation of environment propitious to sludge reduction in bulk sludge. AP₂₅ tended to enrich hydrolytic, fermentative and denitrifying bacteria to accelerate hydrolysis process, while excessive carriers had negative effect on biomass stability and movement of carriers.