Ultrastructural and immunocytochemical analysis of circulating hemocytes from Cherax quadricarinatus (Von Martens, 1868)

Zeng Wentao; Luo Wen; Zhao Yunlong; Wang Danli; Mi Zhongxiang; Shi Getao

doi:10.18805/ijar.v0iOF.6823

Ultrastructural and immunocytochemical analysis of circulating hemocytes from Cherax quadricarinatus (Von Martens, 1868)

Zeng Wentao, Luo Wen, Zhao Yunlong, Wang Danli, Mi Zhongxiang, Shi Getao

School of Life Sciences

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Transmission electron microscopy (TEM) and immunocytochemistry were used to assay the circulating hemocytes characteristics in Cherax quadricarinatus (von Martens, 1868). Three haemocyte types could be identified in circulating hemocytes by TEM, i.e. hyaline cells (HC), semigranular cells (SGC), or granular cells (GC). The circulating hemocytes from C. quadricarinatus could be separated into layers by density gradient centrifugation. The CD34+ cells which are believed to be stem cell-like cells has been discovered in the first layer via immunocytochemical assay, which has not been reported in previous studies. These CD34+ cells may be immature cells derived from a single undifferentiated precursor cell line in specialized hematopoietic tissue (HPT).

Original language	English
Pages (from-to)	129-134
Number of pages	6
Journal	Indian Journal of Animal Research
Volume	51
Issue number	1
DOIs	https://doi.org/10.18805/ijar.v0iOF.6823
State	Published - Feb 2017

Keywords

CD34+ cells
Circulating hemolytic
Density gradient centrifugation

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10.18805/ijar.v0iOF.6823

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abstract = "Transmission electron microscopy (TEM) and immunocytochemistry were used to assay the circulating hemocytes characteristics in Cherax quadricarinatus (von Martens, 1868). Three haemocyte types could be identified in circulating hemocytes by TEM, i.e. hyaline cells (HC), semigranular cells (SGC), or granular cells (GC). The circulating hemocytes from C. quadricarinatus could be separated into layers by density gradient centrifugation. The CD34+ cells which are believed to be stem cell-like cells has been discovered in the first layer via immunocytochemical assay, which has not been reported in previous studies. These CD34+ cells may be immature cells derived from a single undifferentiated precursor cell line in specialized hematopoietic tissue (HPT).",

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author = "Zeng Wentao and Luo Wen and Zhao Yunlong and Wang Danli and Mi Zhongxiang and Shi Getao",

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T1 - Ultrastructural and immunocytochemical analysis of circulating hemocytes from Cherax quadricarinatus (Von Martens, 1868)

AU - Wentao, Zeng

AU - Wen, Luo

AU - Yunlong, Zhao

AU - Danli, Wang

AU - Zhongxiang, Mi

AU - Getao, Shi

PY - 2017/2

Y1 - 2017/2

N2 - Transmission electron microscopy (TEM) and immunocytochemistry were used to assay the circulating hemocytes characteristics in Cherax quadricarinatus (von Martens, 1868). Three haemocyte types could be identified in circulating hemocytes by TEM, i.e. hyaline cells (HC), semigranular cells (SGC), or granular cells (GC). The circulating hemocytes from C. quadricarinatus could be separated into layers by density gradient centrifugation. The CD34+ cells which are believed to be stem cell-like cells has been discovered in the first layer via immunocytochemical assay, which has not been reported in previous studies. These CD34+ cells may be immature cells derived from a single undifferentiated precursor cell line in specialized hematopoietic tissue (HPT).

AB - Transmission electron microscopy (TEM) and immunocytochemistry were used to assay the circulating hemocytes characteristics in Cherax quadricarinatus (von Martens, 1868). Three haemocyte types could be identified in circulating hemocytes by TEM, i.e. hyaline cells (HC), semigranular cells (SGC), or granular cells (GC). The circulating hemocytes from C. quadricarinatus could be separated into layers by density gradient centrifugation. The CD34+ cells which are believed to be stem cell-like cells has been discovered in the first layer via immunocytochemical assay, which has not been reported in previous studies. These CD34+ cells may be immature cells derived from a single undifferentiated precursor cell line in specialized hematopoietic tissue (HPT).

KW - CD34+ cells

KW - Circulating hemolytic

KW - Density gradient centrifugation

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DO - 10.18805/ijar.v0iOF.6823

M3 - 文章

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SN - 0367-6722

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JO - Indian Journal of Animal Research

JF - Indian Journal of Animal Research

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Ultrastructural and immunocytochemical analysis of circulating hemocytes from Cherax quadricarinatus (Von Martens, 1868)

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