Ultrasensitive fluorescence polarization DNA detection by target assisted exonuclease III-catalyzed signal amplification

Min Zhang, Yi Meng Guan, Bang Ce Ye

Research output: Contribution to journalArticlepeer-review

94 Scopus citations

Abstract

Single stranded DNA sequences can be detected by target assisted exonuclease III-catalyzed signal amplification fluorescence polarization (TAECA-FP). The method offers an impressive detection limit of 83 aM within one hour for DNA detection and exhibits high discrimination ability even against a single base mismatch.

Original languageEnglish
Pages (from-to)3478-3480
Number of pages3
JournalChemical Communications
Volume47
Issue number12
DOIs
StatePublished - 8 Mar 2011
Externally publishedYes

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