Abstract
Herein, we describe a novel approach for rapid, label-free and ultrasensitive detection of Hepatitis B virus (HBV) DNA by applying rolling circle amplification (RCA) based on silicon nanowire field-effect transistor (SiNW-FET). Optical lithography and anisotropic self-stop etching were employed to guarantee low cost batch production for SiNWs. The RCA reaction created a long single-stranded DNA (ssDNA) product and thus enhanced the electronic responses of SiNW significantly. The ultrahigh sensitivity for 1 fM HBV DNA detection and high specificity for single-nucleotide polymorphism discrimination were achieved.
| Original language | English |
|---|---|
| Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 2008-2010 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806476 |
| State | Published - 2014 |
| Externally published | Yes |
| Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: 26 Oct 2014 → 30 Oct 2014 |
Publication series
| Name | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|
Conference
| Conference | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|---|
| Country/Territory | United States |
| City | San Antonio |
| Period | 26/10/14 → 30/10/14 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- CMOS-compatible
- Nanosensor
- RCA
- Silicon nanowire
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