Two-photon fluorescence imaging and ratiometric quantification of mitochondrial monoamine oxidase-A in neurons

Yuxiao Mei; Zhichao Liu; Meijun Liu; Jiacheng Gong; Xiao He; Qi Wei Zhang; Yang Tian

doi:10.1039/d2cc01909d

Two-photon fluorescence imaging and ratiometric quantification of mitochondrial monoamine oxidase-A in neurons

Yuxiao Mei
, Zhichao Liu
, Meijun Liu
, Jiacheng Gong
, Xiao He
, Qi Wei Zhang^*
, Yang Tian^*

^*Corresponding author for this work

School of Chemistry and Molecular Engineering

East China Normal University

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Herein, we designed and developed a single two-photon ratiometric fluorescence probe (TMF2P) for selective and accurate determination of mitochondrial MAO-A in live neurons. It was discovered that the increases in MAO-A levels under oxidative stress resulted in an elevated influx of Ca²⁺ flow into mitochondria through the transient receptor potential melastatin 2 (TRPM2) channels.

Original language	English
Pages (from-to)	6657-6660
Number of pages	4
Journal	Chemical Communications
Volume	58
Issue number	46
DOIs	https://doi.org/10.1039/d2cc01909d
State	Published - 14 May 2022

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10.1039/d2cc01909d

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title = "Two-photon fluorescence imaging and ratiometric quantification of mitochondrial monoamine oxidase-A in neurons",

abstract = "Herein, we designed and developed a single two-photon ratiometric fluorescence probe (TMF2P) for selective and accurate determination of mitochondrial MAO-A in live neurons. It was discovered that the increases in MAO-A levels under oxidative stress resulted in an elevated influx of Ca2+ flow into mitochondria through the transient receptor potential melastatin 2 (TRPM2) channels.",

author = "Yuxiao Mei and Zhichao Liu and Meijun Liu and Jiacheng Gong and Xiao He and Zhang, \{Qi Wei\} and Yang Tian",

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T1 - Two-photon fluorescence imaging and ratiometric quantification of mitochondrial monoamine oxidase-A in neurons

AU - Mei, Yuxiao

AU - Liu, Zhichao

AU - Liu, Meijun

AU - Gong, Jiacheng

AU - He, Xiao

AU - Zhang, Qi Wei

AU - Tian, Yang

PY - 2022/5/14

Y1 - 2022/5/14

N2 - Herein, we designed and developed a single two-photon ratiometric fluorescence probe (TMF2P) for selective and accurate determination of mitochondrial MAO-A in live neurons. It was discovered that the increases in MAO-A levels under oxidative stress resulted in an elevated influx of Ca2+ flow into mitochondria through the transient receptor potential melastatin 2 (TRPM2) channels.

AB - Herein, we designed and developed a single two-photon ratiometric fluorescence probe (TMF2P) for selective and accurate determination of mitochondrial MAO-A in live neurons. It was discovered that the increases in MAO-A levels under oxidative stress resulted in an elevated influx of Ca2+ flow into mitochondria through the transient receptor potential melastatin 2 (TRPM2) channels.

UR - https://www.scopus.com/pages/publications/85131771732

U2 - 10.1039/d2cc01909d

DO - 10.1039/d2cc01909d

M3 - 文章

C2 - 35593312

AN - SCOPUS:85131771732

SN - 1359-7345

VL - 58

SP - 6657

EP - 6660

JO - Chemical Communications

JF - Chemical Communications

IS - 46

ER -

Two-photon fluorescence imaging and ratiometric quantification of mitochondrial monoamine oxidase-A in neurons

Abstract

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