Transcriptomic and Metabolomic Signatures of Acute Circadian Misalignment in Mice

Baoyin Ren; Yingzhi Huang; Hui Wang; Haonan Li; Changxiao Ma; Meina Guo; Jiaxin Liu; Hongmei Tan; Jiayang Zhang; Huiwen Zheng; Bingyi Shen; Jiazhi Li; Zhaiyi Liu; Jingwen Zhu; Lejia Qiu; Xiaoyan Zhang; Youfei Guan; Guangrui Yang; Lihong Chen

doi:10.1111/jpi.70075

Transcriptomic and Metabolomic Signatures of Acute Circadian Misalignment in Mice

Baoyin Ren, Yingzhi Huang, Hui Wang, Haonan Li, Changxiao Ma, Meina Guo, Jiaxin Liu, Hongmei Tan, Jiayang Zhang, Huiwen Zheng, Bingyi Shen, Jiazhi Li, Zhaiyi Liu, Jingwen Zhu, Lejia Qiu, Xiaoyan Zhang, Youfei Guan, Guangrui Yang^*, Lihong Chen^*

^*Corresponding author for this work

Health Science Center

Research output: Contribution to journal › Article › peer-review

Abstract

Acute circadian misalignment, such as that induced by a single episode of jet lag, can leave molecular traces even after behavioral rhythms appear to recover. Here, we applied an integrated multi-omics approach—combining liver transcriptomics and plasma metabolomics—to characterize residual signatures on the 7th day after a single 6-h phase advance in male mice. Our data revealed significant alterations, particularly in the core clock genes Bmal1 and Cry1, and the metabolites l-arginine and SM(d18:1/18:1(11Z)), with notable differences at Zeitgeber Time 0 (ZT0). Additionally, we identified l-Serine as a potential biomarker for circadian misalignment, independent of sampling time. These findings provide new insights into potential biomarkers for detecting acute circadian misalignment.

Original language	English
Article number	e70075
Journal	Journal of Pineal Research
Volume	77
Issue number	5
DOIs	https://doi.org/10.1111/jpi.70075
State	Published - Sep 2025

Keywords

Bmal1
biomarker
circadian misalignment
metabolomics
transcriptomics

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10.1111/jpi.70075

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title = "Transcriptomic and Metabolomic Signatures of Acute Circadian Misalignment in Mice",

abstract = "Acute circadian misalignment, such as that induced by a single episode of jet lag, can leave molecular traces even after behavioral rhythms appear to recover. Here, we applied an integrated multi-omics approach—combining liver transcriptomics and plasma metabolomics—to characterize residual signatures on the 7th day after a single 6-h phase advance in male mice. Our data revealed significant alterations, particularly in the core clock genes Bmal1 and Cry1, and the metabolites l-arginine and SM(d18:1/18:1(11Z)), with notable differences at Zeitgeber Time 0 (ZT0). Additionally, we identified l-Serine as a potential biomarker for circadian misalignment, independent of sampling time. These findings provide new insights into potential biomarkers for detecting acute circadian misalignment.",

keywords = "Bmal1, biomarker, circadian misalignment, metabolomics, transcriptomics",

author = "Baoyin Ren and Yingzhi Huang and Hui Wang and Haonan Li and Changxiao Ma and Meina Guo and Jiaxin Liu and Hongmei Tan and Jiayang Zhang and Huiwen Zheng and Bingyi Shen and Jiazhi Li and Zhaiyi Liu and Jingwen Zhu and Lejia Qiu and Xiaoyan Zhang and Youfei Guan and Guangrui Yang and Lihong Chen",

note = "Publisher Copyright: {\textcopyright} 2025 John Wiley \& Sons A/S. Published by John Wiley \& Sons Ltd.",

year = "2025",

month = sep,

doi = "10.1111/jpi.70075",

language = "英语",

volume = "77",

journal = "Journal of Pineal Research",

issn = "0742-3098",

publisher = "Wiley-Blackwell Publishing Ltd",

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TY - JOUR

T1 - Transcriptomic and Metabolomic Signatures of Acute Circadian Misalignment in Mice

AU - Ren, Baoyin

AU - Huang, Yingzhi

AU - Wang, Hui

AU - Li, Haonan

AU - Ma, Changxiao

AU - Guo, Meina

AU - Liu, Jiaxin

AU - Tan, Hongmei

AU - Zhang, Jiayang

AU - Zheng, Huiwen

AU - Shen, Bingyi

AU - Li, Jiazhi

AU - Liu, Zhaiyi

AU - Zhu, Jingwen

AU - Qiu, Lejia

AU - Zhang, Xiaoyan

AU - Guan, Youfei

AU - Yang, Guangrui

AU - Chen, Lihong

PY - 2025/9

Y1 - 2025/9

N2 - Acute circadian misalignment, such as that induced by a single episode of jet lag, can leave molecular traces even after behavioral rhythms appear to recover. Here, we applied an integrated multi-omics approach—combining liver transcriptomics and plasma metabolomics—to characterize residual signatures on the 7th day after a single 6-h phase advance in male mice. Our data revealed significant alterations, particularly in the core clock genes Bmal1 and Cry1, and the metabolites l-arginine and SM(d18:1/18:1(11Z)), with notable differences at Zeitgeber Time 0 (ZT0). Additionally, we identified l-Serine as a potential biomarker for circadian misalignment, independent of sampling time. These findings provide new insights into potential biomarkers for detecting acute circadian misalignment.

AB - Acute circadian misalignment, such as that induced by a single episode of jet lag, can leave molecular traces even after behavioral rhythms appear to recover. Here, we applied an integrated multi-omics approach—combining liver transcriptomics and plasma metabolomics—to characterize residual signatures on the 7th day after a single 6-h phase advance in male mice. Our data revealed significant alterations, particularly in the core clock genes Bmal1 and Cry1, and the metabolites l-arginine and SM(d18:1/18:1(11Z)), with notable differences at Zeitgeber Time 0 (ZT0). Additionally, we identified l-Serine as a potential biomarker for circadian misalignment, independent of sampling time. These findings provide new insights into potential biomarkers for detecting acute circadian misalignment.

KW - Bmal1

KW - biomarker

KW - circadian misalignment

KW - metabolomics

KW - transcriptomics

UR - https://www.scopus.com/pages/publications/105015105159

U2 - 10.1111/jpi.70075

DO - 10.1111/jpi.70075

M3 - 文章

AN - SCOPUS:105015105159

SN - 0742-3098

VL - 77

JO - Journal of Pineal Research

JF - Journal of Pineal Research

IS - 5

M1 - e70075

ER -

Transcriptomic and Metabolomic Signatures of Acute Circadian Misalignment in Mice

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Keywords

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