The screening of interaction factors with BoCAL and BoAP1 related to curd formation

CAL (CAULIFLOWER) gene and AP1 (APETALA1) gene, which both belong to MADS-box transcription factors, are involved in the development of flower apical meristem. In A rabidopsis thaliana, loss of CAL and AP1 function at the same time leads to floweral meristems proliferation indeterminately, and the plants display the cauliflower phenotype. But in cauliflower (Brasscia oleracea L. var. botrytis), the BobCAL single mutant can display the cauliflower phenotype, it is appear that CAL and AP1 homolog have different function in both plants. To study the function of homozygous CAL , AP1 protein in Brassica Species, especially their regulation function in the formation of cauliflower, we took advantage of yeast-two-hybrid method to screen interaction factors of BoCAL. We got four kinds of proteins which are separately involve in phosphorylation and dephosphorylation of proteins, proteins modifying, proteins binding-site and so on. They separately have close relationship with translation, regulation pathways and signal transferring pathways and they provide clues for the study of function of BoCAL. Additionally, we checked up the relationship between some interacting factors of BoCAL and BoAP1, some known MADS-box translation factors and BoCAL and BoAP1. The results showed that BoCAL specifically interact with SnRKbeta2. BoCAL, BoAP1 both interact with SVP, similar as the homolog in Arabidopsis. While the interaction between BoCAL, BoAP1 with FLM, SOC1 and AGL24. The data here indicate the function of homozygous BoCAL and BoAP1 differ from that in Arabidopsis thaliana.