The novel pillar[5]arene derivative for recyclable electrochemical sensing platform of homogeneous DNA hybridization

Shuai Yang; Lijing Liu; Min You; Fan Zhang; Xiaojuan Liao; Pingang He

doi:10.1016/j.snb.2015.12.090

The novel pillar[5]arene derivative for recyclable electrochemical sensing platform of homogeneous DNA hybridization

Shuai Yang
, Lijing Liu
, Min You
, Fan Zhang
, Xiaojuan Liao^*
, Pingang He

^*Corresponding author for this work

School of Chemistry and Molecular Engineering

East China Normal University

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

In this work, a recyclable electrochemical sensing platform to detect breast cancer susceptibility gene (BRCA) was constructed by pillararenes based host-guest recognition and homogeneous DNA hybridization. BRCA target DNA (T-DNA) formed sandwich-type DNA via homogeneous hybridization with methylene blue labeled signal DNA and alkylamino modified capture DNA, which could form complexes with pillararenes. Such sandwich-type DNA was captured by a novel trithiocarbonate modified pillar[5]arene (P5A-CTA) which was immobilized on the Au electrode. With the help of enzyme amplification, the electrochemical detection signal of target DNA in sensing platform was apparently amplified. This sensing platform exhibited wide linear range and excellent specificity, and was particularly recyclable after simple washing with hot acetonitrile due to the reversible host-guest complexation between P5A-CTA and alkylamino modified DNA.

Original language	English
Pages (from-to)	497-503
Number of pages	7
Journal	Sensors and Actuators B: Chemical
Volume	227
DOIs	https://doi.org/10.1016/j.snb.2015.12.090
State	Published - May 2016

Keywords

DNA detection
Electrochemical
Host-guest chemistry
Pillararenes
Sensing platform

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.snb.2015.12.090

Cite this

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title = "The novel pillar[5]arene derivative for recyclable electrochemical sensing platform of homogeneous DNA hybridization",

abstract = "In this work, a recyclable electrochemical sensing platform to detect breast cancer susceptibility gene (BRCA) was constructed by pillararenes based host-guest recognition and homogeneous DNA hybridization. BRCA target DNA (T-DNA) formed sandwich-type DNA via homogeneous hybridization with methylene blue labeled signal DNA and alkylamino modified capture DNA, which could form complexes with pillararenes. Such sandwich-type DNA was captured by a novel trithiocarbonate modified pillar[5]arene (P5A-CTA) which was immobilized on the Au electrode. With the help of enzyme amplification, the electrochemical detection signal of target DNA in sensing platform was apparently amplified. This sensing platform exhibited wide linear range and excellent specificity, and was particularly recyclable after simple washing with hot acetonitrile due to the reversible host-guest complexation between P5A-CTA and alkylamino modified DNA.",

keywords = "DNA detection, Electrochemical, Host-guest chemistry, Pillararenes, Sensing platform",

author = "Shuai Yang and Lijing Liu and Min You and Fan Zhang and Xiaojuan Liao and Pingang He",

year = "2016",

month = may,

doi = "10.1016/j.snb.2015.12.090",

language = "英语",

volume = "227",

pages = "497--503",

journal = "Sensors and Actuators B: Chemical",

issn = "0925-4005",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - The novel pillar[5]arene derivative for recyclable electrochemical sensing platform of homogeneous DNA hybridization

AU - Yang, Shuai

AU - Liu, Lijing

AU - You, Min

AU - Zhang, Fan

AU - Liao, Xiaojuan

AU - He, Pingang

PY - 2016/5

Y1 - 2016/5

N2 - In this work, a recyclable electrochemical sensing platform to detect breast cancer susceptibility gene (BRCA) was constructed by pillararenes based host-guest recognition and homogeneous DNA hybridization. BRCA target DNA (T-DNA) formed sandwich-type DNA via homogeneous hybridization with methylene blue labeled signal DNA and alkylamino modified capture DNA, which could form complexes with pillararenes. Such sandwich-type DNA was captured by a novel trithiocarbonate modified pillar[5]arene (P5A-CTA) which was immobilized on the Au electrode. With the help of enzyme amplification, the electrochemical detection signal of target DNA in sensing platform was apparently amplified. This sensing platform exhibited wide linear range and excellent specificity, and was particularly recyclable after simple washing with hot acetonitrile due to the reversible host-guest complexation between P5A-CTA and alkylamino modified DNA.

AB - In this work, a recyclable electrochemical sensing platform to detect breast cancer susceptibility gene (BRCA) was constructed by pillararenes based host-guest recognition and homogeneous DNA hybridization. BRCA target DNA (T-DNA) formed sandwich-type DNA via homogeneous hybridization with methylene blue labeled signal DNA and alkylamino modified capture DNA, which could form complexes with pillararenes. Such sandwich-type DNA was captured by a novel trithiocarbonate modified pillar[5]arene (P5A-CTA) which was immobilized on the Au electrode. With the help of enzyme amplification, the electrochemical detection signal of target DNA in sensing platform was apparently amplified. This sensing platform exhibited wide linear range and excellent specificity, and was particularly recyclable after simple washing with hot acetonitrile due to the reversible host-guest complexation between P5A-CTA and alkylamino modified DNA.

KW - DNA detection

KW - Electrochemical

KW - Host-guest chemistry

KW - Pillararenes

KW - Sensing platform

UR - https://www.scopus.com/pages/publications/84953725626

U2 - 10.1016/j.snb.2015.12.090

DO - 10.1016/j.snb.2015.12.090

M3 - 文章

AN - SCOPUS:84953725626

SN - 0925-4005

VL - 227

SP - 497

EP - 503

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

ER -

The novel pillar[5]arene derivative for recyclable electrochemical sensing platform of homogeneous DNA hybridization

Abstract

Keywords

UN SDGs

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