The enzyme-amplified amperometric DNA sensor using an electrodeposited polymer redox mediator

Lanyong Zhang; Ying Wan; Jiong Zhang; Di Li; Lihua Wang; Shiping Song; Chunhai Fan

doi:10.1007/s11426-009-0084-1

The enzyme-amplified amperometric DNA sensor using an electrodeposited polymer redox mediator

Lanyong Zhang, Ying Wan, Jiong Zhang, Di Li, Lihua Wang, Shiping Song, Chunhai Fan

Chinese Academy of Sciences

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A highly sensitive method for the detection of a breast cancer-associated BRCA-1 gene is reported. The detection is based on a classical sandwich-type assay using horseradish peroxidase (HRP) as a catalytic label and electrodeposited Os^2+/3+ conducting polymer (PAA-PVI-Os) as a redox mediator. Target DNA could be detected by the HRP-catalyzed reduction of H ₂O₂, leading to a limit of detection as low as 10 fM.

Original language	English
Pages (from-to)	746-750
Number of pages	5
Journal	Science in China, Series B: Chemistry
Volume	52
Issue number	6
DOIs	https://doi.org/10.1007/s11426-009-0084-1
State	Published - Jun 2009
Externally published	Yes

Keywords

Conducting polymer
DNA detection
Electrochemistry
HRP

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s11426-009-0084-1

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title = "The enzyme-amplified amperometric DNA sensor using an electrodeposited polymer redox mediator",

abstract = "A highly sensitive method for the detection of a breast cancer-associated BRCA-1 gene is reported. The detection is based on a classical sandwich-type assay using horseradish peroxidase (HRP) as a catalytic label and electrodeposited Os2+/3+ conducting polymer (PAA-PVI-Os) as a redox mediator. Target DNA could be detected by the HRP-catalyzed reduction of H 2O2, leading to a limit of detection as low as 10 fM.",

keywords = "Conducting polymer, DNA detection, Electrochemistry, HRP",

author = "Lanyong Zhang and Ying Wan and Jiong Zhang and Di Li and Lihua Wang and Shiping Song and Chunhai Fan",

year = "2009",

month = jun,

doi = "10.1007/s11426-009-0084-1",

language = "英语",

volume = "52",

pages = "746--750",

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TY - JOUR

T1 - The enzyme-amplified amperometric DNA sensor using an electrodeposited polymer redox mediator

AU - Zhang, Lanyong

AU - Wan, Ying

AU - Zhang, Jiong

AU - Li, Di

AU - Wang, Lihua

AU - Song, Shiping

AU - Fan, Chunhai

PY - 2009/6

Y1 - 2009/6

N2 - A highly sensitive method for the detection of a breast cancer-associated BRCA-1 gene is reported. The detection is based on a classical sandwich-type assay using horseradish peroxidase (HRP) as a catalytic label and electrodeposited Os2+/3+ conducting polymer (PAA-PVI-Os) as a redox mediator. Target DNA could be detected by the HRP-catalyzed reduction of H 2O2, leading to a limit of detection as low as 10 fM.

AB - A highly sensitive method for the detection of a breast cancer-associated BRCA-1 gene is reported. The detection is based on a classical sandwich-type assay using horseradish peroxidase (HRP) as a catalytic label and electrodeposited Os2+/3+ conducting polymer (PAA-PVI-Os) as a redox mediator. Target DNA could be detected by the HRP-catalyzed reduction of H 2O2, leading to a limit of detection as low as 10 fM.

KW - Conducting polymer

KW - DNA detection

KW - Electrochemistry

KW - HRP

UR - https://www.scopus.com/pages/publications/65949112934

U2 - 10.1007/s11426-009-0084-1

DO - 10.1007/s11426-009-0084-1

M3 - 文章

AN - SCOPUS:65949112934

SN - 1006-9291

VL - 52

SP - 746

EP - 750

JO - Science in China, Series B: Chemistry

JF - Science in China, Series B: Chemistry

IS - 6

ER -

The enzyme-amplified amperometric DNA sensor using an electrodeposited polymer redox mediator

Abstract

Keywords

UN SDGs

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