The determination of monoamine transmitters and their metabolites in vivo by microdialysis using high performance liquid chromatography with nickel hexacyanoferrate chemically modified electrode

In this paper, we prepared a nickel hexacyanoferrate(NiHCF) chemically modified electrode (CME) and discussed its mechanism cf the electrocatalytic oxidation to monoamine transmitters and their metabolites. The separation was performed on a C₁₈ column with an eluting system containing phosphate buffer (pH = 5.0) and methanol. A good linear relationship was found between the concentration and peak heights of the monoamine transmitters and their metabolites from 10^-8 mol/L to 10^-5 mol/L. We coupled microdialysis sampling technique with LC and determined the monoamine transmitters and their metabolites of caudatum in freely moving rat. At the same time, we also studied the effect of Ca²⁺ on the transimmitters.