The complete mitochondrial genome of the king horseshoe bat (Rhinolophus rex) using next-generation sequencing and Sanger sequencing

Huizhen Shi; Shuyi Zhang; Xiuguang Mao

doi:10.3109/19401736.2015.1101555

The complete mitochondrial genome of the king horseshoe bat (Rhinolophus rex) using next-generation sequencing and Sanger sequencing

Huizhen Shi, Shuyi Zhang, Xiuguang Mao

School of Ecology and Environmental Sciences

East China Normal University

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The king horseshoe bat (Rhinolophus rex) is endemic to China. Here, we characterized the complete mitochondrial genome of R. rex using next-generation sequencing and Sanger sequencing. The mitogenome is 16 845 bp in length and contains 22 tRNAs, two rRNAs, 13 protein-coding genes, and a non-coding control region. Our traditional Sanger sequencing verified that mtDNA sequences generated from next-generation sequencing are reliable. The mitogenome of R. rex will be useful for the conservation genetics of this endangered species.

Original language	English
Pages (from-to)	4545-4546
Number of pages	2
Journal	Mitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis
Volume	27
Issue number	6
DOIs	https://doi.org/10.3109/19401736.2015.1101555
State	Published - 1 Nov 2016

Keywords

Bat
Rhinolophus rex
de novo assembly
mitogenome

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10.3109/19401736.2015.1101555

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abstract = "The king horseshoe bat (Rhinolophus rex) is endemic to China. Here, we characterized the complete mitochondrial genome of R. rex using next-generation sequencing and Sanger sequencing. The mitogenome is 16 845 bp in length and contains 22 tRNAs, two rRNAs, 13 protein-coding genes, and a non-coding control region. Our traditional Sanger sequencing verified that mtDNA sequences generated from next-generation sequencing are reliable. The mitogenome of R. rex will be useful for the conservation genetics of this endangered species.",

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AU - Mao, Xiuguang

PY - 2016/11/1

Y1 - 2016/11/1

N2 - The king horseshoe bat (Rhinolophus rex) is endemic to China. Here, we characterized the complete mitochondrial genome of R. rex using next-generation sequencing and Sanger sequencing. The mitogenome is 16 845 bp in length and contains 22 tRNAs, two rRNAs, 13 protein-coding genes, and a non-coding control region. Our traditional Sanger sequencing verified that mtDNA sequences generated from next-generation sequencing are reliable. The mitogenome of R. rex will be useful for the conservation genetics of this endangered species.

AB - The king horseshoe bat (Rhinolophus rex) is endemic to China. Here, we characterized the complete mitochondrial genome of R. rex using next-generation sequencing and Sanger sequencing. The mitogenome is 16 845 bp in length and contains 22 tRNAs, two rRNAs, 13 protein-coding genes, and a non-coding control region. Our traditional Sanger sequencing verified that mtDNA sequences generated from next-generation sequencing are reliable. The mitogenome of R. rex will be useful for the conservation genetics of this endangered species.

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KW - Rhinolophus rex

KW - de novo assembly

KW - mitogenome

UR - https://www.scopus.com/pages/publications/85003545572

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JO - Mitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis

JF - Mitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis

IS - 6

ER -

The complete mitochondrial genome of the king horseshoe bat (Rhinolophus rex) using next-generation sequencing and Sanger sequencing

Abstract

Keywords

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