TY - JOUR
T1 - The bio-functional role of calcium in mesoporous silica xerogels on the responses of osteoblasts in vitro
AU - Zhou, Huanjun
AU - Wei, Jie
AU - Wu, Xiaohui
AU - Shi, Jianlin
AU - Liu, Changsheng
AU - Jia, Junfeng
AU - Dai, Chenglong
AU - Gan, Qi
PY - 2010/7
Y1 - 2010/7
N2 - Mesoporous silica xerogels with various amount of calcium (0, 5, 10 and 15%, named m-SXC0, m-SXC5, m-SXC10 and m-SXC15, respectively) were synthesized by template sol-gel methods, and cell responses to m-SXCs were studied using murine pre-osteoblast MC3T3-E1 in vitro. The results showed that cell morphology was not affected by m-SXCs indicating good biocompatibility. Furthermore, cell proliferation ratio on the m-SXCs increased over time, among which m-SXC10 was highest. NO production obviously rose with the increase of Ca content in m-SXCs. ALP activity and PGE2 level on m-SXC5 significantly improved compared with m-SXC0 while decreased with the increase of Ca content for m-SXC10 and m-SXC15. No obvious discrepancy on osteopontin mRNA expressions was observed among m-SXCs. The collagen I and osteocalcin mRNA expression on m-SXC5 were up-regulated, while decreased on m-SXC15 evidently. The phosphorylation level of ERK 1/2 for the m-SXC10 was highest after 7 days. In conclusion, calcium in m-SXCs plays an important role in osteoblast activity, which indicates mesoporous silica xerogel containing appropriate calcium could stimulate osteoblast proliferation, differentiation, gene expression via the activation of ERK 1/2 signaling pathway, and shows great prospects in bone regeneration field using as a drug controlled release filler.
AB - Mesoporous silica xerogels with various amount of calcium (0, 5, 10 and 15%, named m-SXC0, m-SXC5, m-SXC10 and m-SXC15, respectively) were synthesized by template sol-gel methods, and cell responses to m-SXCs were studied using murine pre-osteoblast MC3T3-E1 in vitro. The results showed that cell morphology was not affected by m-SXCs indicating good biocompatibility. Furthermore, cell proliferation ratio on the m-SXCs increased over time, among which m-SXC10 was highest. NO production obviously rose with the increase of Ca content in m-SXCs. ALP activity and PGE2 level on m-SXC5 significantly improved compared with m-SXC0 while decreased with the increase of Ca content for m-SXC10 and m-SXC15. No obvious discrepancy on osteopontin mRNA expressions was observed among m-SXCs. The collagen I and osteocalcin mRNA expression on m-SXC5 were up-regulated, while decreased on m-SXC15 evidently. The phosphorylation level of ERK 1/2 for the m-SXC10 was highest after 7 days. In conclusion, calcium in m-SXCs plays an important role in osteoblast activity, which indicates mesoporous silica xerogel containing appropriate calcium could stimulate osteoblast proliferation, differentiation, gene expression via the activation of ERK 1/2 signaling pathway, and shows great prospects in bone regeneration field using as a drug controlled release filler.
UR - https://www.scopus.com/pages/publications/77954542635
U2 - 10.1007/s10856-010-4083-8
DO - 10.1007/s10856-010-4083-8
M3 - 文章
C2 - 20411307
AN - SCOPUS:77954542635
SN - 0957-4530
VL - 21
SP - 2175
EP - 2185
JO - Journal of Materials Science: Materials in Medicine
JF - Journal of Materials Science: Materials in Medicine
IS - 7
ER -