TY - JOUR
T1 - Terminal deoxynucleotidyl transferase (TdT)-catalyzed homo-nucleotides-constituted ssDNA
T2 - Inducing tunable-size nanogap for core-shell plasmonic metal nanostructure and acting as Raman reporters for detection of Escherichia coli O157:H7
AU - Zhou, Yangyang
AU - Fang, Weina
AU - Lai, Keqiang
AU - Zhu, Yongheng
AU - Bian, Xiaojun
AU - Shen, Jianlei
AU - Li, Qian
AU - Wang, Lihua
AU - Zhang, Weijia
AU - Yan, Juan
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/9/15
Y1 - 2019/9/15
N2 - Core-shell plasmonic metal nanoparticles with interior nanogaps are superior nanostructures owing to their large signal enhancement for Surface enhanced Raman spectroscopy (SERS). Herein, we incorporated Terminal deoxynucleotidyl transferase (TdT)-catalyzed DNA in the preparation of core-shell nanostructures for the detection of Escherichia coli O157:H7 (E. coli O157:H7) cells. The elongated products-homo-nucleotides-composed of long single DNA strands (hn-D) are used not only to induce tunable-size nanogaps but also as Raman reporters with consistent and uniform signal enhancement. Using this synthetic process of hn-D-embedded core-shell nanoparticles (hn-DENPs), we found that the length of hn-D strands affects the size of the nanogap. In addition, performances of the specific Raman imaging of E. coli O157:H7, high detection sensitivity of 2 CFU/mL, and the recovery of 98.1%–105.2% measured in the real food samples, make hn-DENP a biosensor that will be widely used in biological detection.
AB - Core-shell plasmonic metal nanoparticles with interior nanogaps are superior nanostructures owing to their large signal enhancement for Surface enhanced Raman spectroscopy (SERS). Herein, we incorporated Terminal deoxynucleotidyl transferase (TdT)-catalyzed DNA in the preparation of core-shell nanostructures for the detection of Escherichia coli O157:H7 (E. coli O157:H7) cells. The elongated products-homo-nucleotides-composed of long single DNA strands (hn-D) are used not only to induce tunable-size nanogaps but also as Raman reporters with consistent and uniform signal enhancement. Using this synthetic process of hn-D-embedded core-shell nanoparticles (hn-DENPs), we found that the length of hn-D strands affects the size of the nanogap. In addition, performances of the specific Raman imaging of E. coli O157:H7, high detection sensitivity of 2 CFU/mL, and the recovery of 98.1%–105.2% measured in the real food samples, make hn-DENP a biosensor that will be widely used in biological detection.
KW - Bacterial detection
KW - Core-shell nanostructures
KW - Nanogap
KW - Surface enhanced Raman spectroscopy (SERS)
KW - Terminal deoxynucleotidyl transferase (TdT)
UR - https://www.scopus.com/pages/publications/85067201130
U2 - 10.1016/j.bios.2019.111419
DO - 10.1016/j.bios.2019.111419
M3 - 文章
C2 - 31203177
AN - SCOPUS:85067201130
SN - 0956-5663
VL - 141
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
M1 - 111419
ER -