Targeting cancer cells with biotin-dendrimer conjugates

Wenjun Yang; Yiyun Cheng; Tongwen Xu; Xueyuan Wang; Long ping Wen

doi:10.1016/j.ejmech.2008.04.021

Targeting cancer cells with biotin-dendrimer conjugates

Wenjun Yang
, Yiyun Cheng^*
, Tongwen Xu
, Xueyuan Wang
, Long ping Wen

^*Corresponding author for this work

University of Science and Technology of China

Research output: Contribution to journal › Article › peer-review

287 Scopus citations

Abstract

Star-burst dendrimers represent a superior carrier platform for targeted drug delivery. Partially acetylated generation 5 (G5) polyamidoamine (PAMAM) dendrimer was conjugated with the targeting moiety (biotin) and the imaging moiety (fluoresceinisothiocyanate, FITC), and the resulting dendrimer-biotin conjugate was characterized by ¹H NMR, UV-vis spectrum. As revealed by flow cytometry and confocal microscopy, the bifunctional conjugate (dendrimer-biotin-FITC) exhibited much higher cellular uptake into HeLa cells than the conjugate without biotin. The uptake was energy-dependent, dose-dependent, and could be effectively blocked by dendrimer-conjugated biotin. Our results indicated that the biocompatible biotin-dendrimer conjugate might be a promising nano-platform for cancer therapy and cancer diagnosis.

Original language	English
Pages (from-to)	862-868
Number of pages	7
Journal	European Journal of Medicinal Chemistry
Volume	44
Issue number	2
DOIs	https://doi.org/10.1016/j.ejmech.2008.04.021
State	Published - Feb 2009
Externally published	Yes

Keywords

Biotin
Cancer targeting
Conjugate
Dendrimer
Nano-carrier

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ejmech.2008.04.021

Cite this

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title = "Targeting cancer cells with biotin-dendrimer conjugates",

abstract = "Star-burst dendrimers represent a superior carrier platform for targeted drug delivery. Partially acetylated generation 5 (G5) polyamidoamine (PAMAM) dendrimer was conjugated with the targeting moiety (biotin) and the imaging moiety (fluoresceinisothiocyanate, FITC), and the resulting dendrimer-biotin conjugate was characterized by 1H NMR, UV-vis spectrum. As revealed by flow cytometry and confocal microscopy, the bifunctional conjugate (dendrimer-biotin-FITC) exhibited much higher cellular uptake into HeLa cells than the conjugate without biotin. The uptake was energy-dependent, dose-dependent, and could be effectively blocked by dendrimer-conjugated biotin. Our results indicated that the biocompatible biotin-dendrimer conjugate might be a promising nano-platform for cancer therapy and cancer diagnosis.",

keywords = "Biotin, Cancer targeting, Conjugate, Dendrimer, Nano-carrier",

author = "Wenjun Yang and Yiyun Cheng and Tongwen Xu and Xueyuan Wang and Wen, \{Long ping\}",

year = "2009",

month = feb,

doi = "10.1016/j.ejmech.2008.04.021",

language = "英语",

volume = "44",

pages = "862--868",

journal = "European Journal of Medicinal Chemistry",

issn = "0223-5234",

publisher = "Elsevier Masson s.r.l.",

number = "2",

}

TY - JOUR

T1 - Targeting cancer cells with biotin-dendrimer conjugates

AU - Yang, Wenjun

AU - Cheng, Yiyun

AU - Xu, Tongwen

AU - Wang, Xueyuan

AU - Wen, Long ping

PY - 2009/2

Y1 - 2009/2

N2 - Star-burst dendrimers represent a superior carrier platform for targeted drug delivery. Partially acetylated generation 5 (G5) polyamidoamine (PAMAM) dendrimer was conjugated with the targeting moiety (biotin) and the imaging moiety (fluoresceinisothiocyanate, FITC), and the resulting dendrimer-biotin conjugate was characterized by 1H NMR, UV-vis spectrum. As revealed by flow cytometry and confocal microscopy, the bifunctional conjugate (dendrimer-biotin-FITC) exhibited much higher cellular uptake into HeLa cells than the conjugate without biotin. The uptake was energy-dependent, dose-dependent, and could be effectively blocked by dendrimer-conjugated biotin. Our results indicated that the biocompatible biotin-dendrimer conjugate might be a promising nano-platform for cancer therapy and cancer diagnosis.

AB - Star-burst dendrimers represent a superior carrier platform for targeted drug delivery. Partially acetylated generation 5 (G5) polyamidoamine (PAMAM) dendrimer was conjugated with the targeting moiety (biotin) and the imaging moiety (fluoresceinisothiocyanate, FITC), and the resulting dendrimer-biotin conjugate was characterized by 1H NMR, UV-vis spectrum. As revealed by flow cytometry and confocal microscopy, the bifunctional conjugate (dendrimer-biotin-FITC) exhibited much higher cellular uptake into HeLa cells than the conjugate without biotin. The uptake was energy-dependent, dose-dependent, and could be effectively blocked by dendrimer-conjugated biotin. Our results indicated that the biocompatible biotin-dendrimer conjugate might be a promising nano-platform for cancer therapy and cancer diagnosis.

KW - Biotin

KW - Cancer targeting

KW - Conjugate

KW - Dendrimer

KW - Nano-carrier

UR - https://www.scopus.com/pages/publications/60149100143

U2 - 10.1016/j.ejmech.2008.04.021

DO - 10.1016/j.ejmech.2008.04.021

M3 - 文章

C2 - 18550227

AN - SCOPUS:60149100143

SN - 0223-5234

VL - 44

SP - 862

EP - 868

JO - European Journal of Medicinal Chemistry

JF - European Journal of Medicinal Chemistry

IS - 2

ER -

Targeting cancer cells with biotin-dendrimer conjugates

Abstract

Keywords

UN SDGs

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