Synthetic peptides derived from SARS coronavirus S protein with diagnostic and therapeutic potential

Wei Lu; Xiao Dong Wu; Mu De Shi; Rui Fu Yang; You Yu He; Chao Bian; Tie Liu Shi; Sheng Yang; Xue Liang Zhu; Wei Hong Jiang; Yi Xue Li; Lin Chen Yan; Yong Yong Ji; Ying Lin; Guo Mei Lin; Lin Tian; Jin Wang; Hong Xia Wang; You Hua Xie; Gang Pei; Jia Rui Wu; Bing Sun

doi:10.1016/j.febslet.2005.02.070

Synthetic peptides derived from SARS coronavirus S protein with diagnostic and therapeutic potential

Wei Lu
, Xiao Dong Wu
, Mu De Shi
, Rui Fu Yang
, You Yu He
, Chao Bian
, Tie Liu Shi
, Sheng Yang
, Xue Liang Zhu
, Wei Hong Jiang
, Yi Xue Li
, Lin Chen Yan
, Yong Yong Ji
, Ying Lin
, Guo Mei Lin
, Lin Tian
, Jin Wang
, Hong Xia Wang
, You Hua Xie
, Gang Pei

Jia Rui Wu^*, Bing Sun

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

The spike (S) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is an important viral structural protein. Based on bioinformatics analysis, 10 antigenic peptides derived from the S protein sequence were selected and synthesized. The antigenicity and immunoreactivity of all the peptides were tested in vivo and in vitro. Four peptides (P6, P8, P9 and P10) which contain B cell epitopes of the S protein were identified, and P8 peptide was confirmed in vivo to have a potential in serological diagnosis. By using a syncytia formation model, we tested the neutralization ability of all 10 peptides and their corresponding antibodies. It is interesting to find that P8 and P9 peptides inhibited syncytia formation, suggesting that the P8 and P9 spanning regions may provide a good target for anti-SARS-CoV drug design. Our data suggest that we have identified peptides derived from the S protein of SARS-CoV, which are useful for SARS treatment and diagnosis.

Original language	English
Pages (from-to)	2130-2136
Number of pages	7
Journal	FEBS Letters
Volume	579
Issue number	10
DOIs	https://doi.org/10.1016/j.febslet.2005.02.070
State	Published - 11 Apr 2005
Externally published	Yes

Keywords

Severe acute respiratory syndrome coronavirus
Spike protein
Syncytia formation and disease prevention
Synthetic peptide

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10.1016/j.febslet.2005.02.070

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Lu, W., Wu, X. D., Shi, M. D., Yang, R. F., He, Y. Y., Bian, C., Shi, T. L., Yang, S., Zhu, X. L., Jiang, W. H., Li, Y. X., Yan, L. C., Ji, Y. Y., Lin, Y., Lin, G. M., Tian, L., Wang, J., Wang, H. X., Xie, Y. H., ... Sun, B. (2005). Synthetic peptides derived from SARS coronavirus S protein with diagnostic and therapeutic potential. FEBS Letters, 579(10), 2130-2136. https://doi.org/10.1016/j.febslet.2005.02.070

@article{d46d0efc19c44e18b1a18d912e0a67a6,

title = "Synthetic peptides derived from SARS coronavirus S protein with diagnostic and therapeutic potential",

abstract = "The spike (S) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is an important viral structural protein. Based on bioinformatics analysis, 10 antigenic peptides derived from the S protein sequence were selected and synthesized. The antigenicity and immunoreactivity of all the peptides were tested in vivo and in vitro. Four peptides (P6, P8, P9 and P10) which contain B cell epitopes of the S protein were identified, and P8 peptide was confirmed in vivo to have a potential in serological diagnosis. By using a syncytia formation model, we tested the neutralization ability of all 10 peptides and their corresponding antibodies. It is interesting to find that P8 and P9 peptides inhibited syncytia formation, suggesting that the P8 and P9 spanning regions may provide a good target for anti-SARS-CoV drug design. Our data suggest that we have identified peptides derived from the S protein of SARS-CoV, which are useful for SARS treatment and diagnosis.",

keywords = "Severe acute respiratory syndrome coronavirus, Spike protein, Syncytia formation and disease prevention, Synthetic peptide",

author = "Wei Lu and Wu, \{Xiao Dong\} and Shi, \{Mu De\} and Yang, \{Rui Fu\} and He, \{You Yu\} and Chao Bian and Shi, \{Tie Liu\} and Sheng Yang and Zhu, \{Xue Liang\} and Jiang, \{Wei Hong\} and Li, \{Yi Xue\} and Yan, \{Lin Chen\} and Ji, \{Yong Yong\} and Ying Lin and Lin, \{Guo Mei\} and Lin Tian and Jin Wang and Wang, \{Hong Xia\} and Xie, \{You Hua\} and Gang Pei and Wu, \{Jia Rui\} and Bing Sun",

year = "2005",

month = apr,

day = "11",

doi = "10.1016/j.febslet.2005.02.070",

language = "英语",

volume = "579",

pages = "2130--2136",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc",

number = "10",

}

Lu, W, Wu, XD, Shi, MD, Yang, RF, He, YY, Bian, C, Shi, TL, Yang, S, Zhu, XL, Jiang, WH, Li, YX, Yan, LC, Ji, YY, Lin, Y, Lin, GM, Tian, L, Wang, J, Wang, HX, Xie, YH, Pei, G, Wu, JR & Sun, B 2005, 'Synthetic peptides derived from SARS coronavirus S protein with diagnostic and therapeutic potential', FEBS Letters, vol. 579, no. 10, pp. 2130-2136. https://doi.org/10.1016/j.febslet.2005.02.070

TY - JOUR

T1 - Synthetic peptides derived from SARS coronavirus S protein with diagnostic and therapeutic potential

AU - Lu, Wei

AU - Wu, Xiao Dong

AU - Shi, Mu De

AU - Yang, Rui Fu

AU - He, You Yu

AU - Bian, Chao

AU - Shi, Tie Liu

AU - Yang, Sheng

AU - Zhu, Xue Liang

AU - Jiang, Wei Hong

AU - Li, Yi Xue

AU - Yan, Lin Chen

AU - Ji, Yong Yong

AU - Lin, Ying

AU - Lin, Guo Mei

AU - Tian, Lin

AU - Wang, Jin

AU - Wang, Hong Xia

AU - Xie, You Hua

AU - Pei, Gang

AU - Wu, Jia Rui

AU - Sun, Bing

PY - 2005/4/11

Y1 - 2005/4/11

N2 - The spike (S) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is an important viral structural protein. Based on bioinformatics analysis, 10 antigenic peptides derived from the S protein sequence were selected and synthesized. The antigenicity and immunoreactivity of all the peptides were tested in vivo and in vitro. Four peptides (P6, P8, P9 and P10) which contain B cell epitopes of the S protein were identified, and P8 peptide was confirmed in vivo to have a potential in serological diagnosis. By using a syncytia formation model, we tested the neutralization ability of all 10 peptides and their corresponding antibodies. It is interesting to find that P8 and P9 peptides inhibited syncytia formation, suggesting that the P8 and P9 spanning regions may provide a good target for anti-SARS-CoV drug design. Our data suggest that we have identified peptides derived from the S protein of SARS-CoV, which are useful for SARS treatment and diagnosis.

AB - The spike (S) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is an important viral structural protein. Based on bioinformatics analysis, 10 antigenic peptides derived from the S protein sequence were selected and synthesized. The antigenicity and immunoreactivity of all the peptides were tested in vivo and in vitro. Four peptides (P6, P8, P9 and P10) which contain B cell epitopes of the S protein were identified, and P8 peptide was confirmed in vivo to have a potential in serological diagnosis. By using a syncytia formation model, we tested the neutralization ability of all 10 peptides and their corresponding antibodies. It is interesting to find that P8 and P9 peptides inhibited syncytia formation, suggesting that the P8 and P9 spanning regions may provide a good target for anti-SARS-CoV drug design. Our data suggest that we have identified peptides derived from the S protein of SARS-CoV, which are useful for SARS treatment and diagnosis.

KW - Severe acute respiratory syndrome coronavirus

KW - Spike protein

KW - Syncytia formation and disease prevention

KW - Synthetic peptide

UR - https://www.scopus.com/pages/publications/20144389770

U2 - 10.1016/j.febslet.2005.02.070

DO - 10.1016/j.febslet.2005.02.070

M3 - 文章

C2 - 15811330

AN - SCOPUS:20144389770

SN - 0014-5793

VL - 579

SP - 2130

EP - 2136

JO - FEBS Letters

JF - FEBS Letters

IS - 10

ER -

Synthetic peptides derived from SARS coronavirus S protein with diagnostic and therapeutic potential

Abstract

Keywords

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