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Synthesis and peroxidase-staining properties of novel water soluble polyhydroxylalkyl benzidine dyes

  • Lingfei Liu
  • , Zhang Yulan
  • , Xuhong Qian*
  • *Corresponding author for this work
  • East China University of Science and Technology
  • Dalian University of Technology

Research output: Contribution to journalArticlepeer-review

Abstract

A series of water soluble N -polyhydroxyalkyl benzidine derivatives was synthesized. The oxidation of glucose-benzidine and fructose-benzidine by iodide was faster than that of benzidine, while N -substituted hydroxylethyl and N -mercaptoethyl benzidines were almost the same as benzidine. It was found that these compounds were able to stain peroxidase well, and compound 2 (fructose-benzidine conjugate) gave the best visualization, which was as good as benzidine.

Original languageEnglish
Pages (from-to)17-21
Number of pages5
JournalDyes and Pigments
Volume60
Issue number1
DOIs
StatePublished - Jan 2004
Externally publishedYes

Keywords

  • Benzidine
  • Fructose
  • Glucose
  • Hydroxylethyl
  • Leukemia
  • Mercaptoethyl
  • Oxidation
  • Peroxidase
  • Staining

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