Synthesis and fluorescent studies of a low molecular weight rotor for living cancer cell imaging

Shulei Zhu; Xumeng Yu; Yang He; Mingliang Ma; Wei Lu

doi:10.1016/j.dyepig.2020.108353

Synthesis and fluorescent studies of a low molecular weight rotor for living cancer cell imaging

Shulei Zhu, Xumeng Yu, Yang He, Mingliang Ma, Wei Lu

East China Normal University

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A fluorescent and low molecular weight rotor (9) based on 1, 8-napthalimide for living cancer cell imaging was designed and synthesized. The effects of solvent polarity and viscosity on the fluorescence spectra of compound 9 were investigated. Rotor 9 exhibited favorable photophysical properties with a large Stokes shift and induced high viscosity sensitivity in a glycerol-ethanol system with an approximately 10-fold increase in emission intensity. The addition of different concentrations of BSA to compound 9 in PBS elicited an 8-fold increase in fluorescence intensity, suggesting superior environmental sensitivity. Fluorescence lifetime measurements also confirmed these results. Furthermore, compound 9 showed time- and concentration-dependent in vitro cellular uptake behavior in MCF-7 cells and other cancer cell lines. CLSM assay of tumor sections from MCF-7 nude mice also confirmed the possibility of tumor imaging.

Original language	English
Article number	108353
Journal	Dyes and Pigments
Volume	178
DOIs	https://doi.org/10.1016/j.dyepig.2020.108353
State	Published - Jul 2020

Keywords

Cancer cell imaging
Fluorescence
Low molecular weight
Molecular rotor

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.dyepig.2020.108353

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title = "Synthesis and fluorescent studies of a low molecular weight rotor for living cancer cell imaging",

abstract = "A fluorescent and low molecular weight rotor (9) based on 1, 8-napthalimide for living cancer cell imaging was designed and synthesized. The effects of solvent polarity and viscosity on the fluorescence spectra of compound 9 were investigated. Rotor 9 exhibited favorable photophysical properties with a large Stokes shift and induced high viscosity sensitivity in a glycerol-ethanol system with an approximately 10-fold increase in emission intensity. The addition of different concentrations of BSA to compound 9 in PBS elicited an 8-fold increase in fluorescence intensity, suggesting superior environmental sensitivity. Fluorescence lifetime measurements also confirmed these results. Furthermore, compound 9 showed time- and concentration-dependent in vitro cellular uptake behavior in MCF-7 cells and other cancer cell lines. CLSM assay of tumor sections from MCF-7 nude mice also confirmed the possibility of tumor imaging.",

keywords = "Cancer cell imaging, Fluorescence, Low molecular weight, Molecular rotor",

author = "Shulei Zhu and Xumeng Yu and Yang He and Mingliang Ma and Wei Lu",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

year = "2020",

month = jul,

doi = "10.1016/j.dyepig.2020.108353",

language = "英语",

volume = "178",

journal = "Dyes and Pigments",

issn = "0143-7208",

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TY - JOUR

T1 - Synthesis and fluorescent studies of a low molecular weight rotor for living cancer cell imaging

AU - Zhu, Shulei

AU - Yu, Xumeng

AU - He, Yang

AU - Ma, Mingliang

AU - Lu, Wei

PY - 2020/7

Y1 - 2020/7

N2 - A fluorescent and low molecular weight rotor (9) based on 1, 8-napthalimide for living cancer cell imaging was designed and synthesized. The effects of solvent polarity and viscosity on the fluorescence spectra of compound 9 were investigated. Rotor 9 exhibited favorable photophysical properties with a large Stokes shift and induced high viscosity sensitivity in a glycerol-ethanol system with an approximately 10-fold increase in emission intensity. The addition of different concentrations of BSA to compound 9 in PBS elicited an 8-fold increase in fluorescence intensity, suggesting superior environmental sensitivity. Fluorescence lifetime measurements also confirmed these results. Furthermore, compound 9 showed time- and concentration-dependent in vitro cellular uptake behavior in MCF-7 cells and other cancer cell lines. CLSM assay of tumor sections from MCF-7 nude mice also confirmed the possibility of tumor imaging.

AB - A fluorescent and low molecular weight rotor (9) based on 1, 8-napthalimide for living cancer cell imaging was designed and synthesized. The effects of solvent polarity and viscosity on the fluorescence spectra of compound 9 were investigated. Rotor 9 exhibited favorable photophysical properties with a large Stokes shift and induced high viscosity sensitivity in a glycerol-ethanol system with an approximately 10-fold increase in emission intensity. The addition of different concentrations of BSA to compound 9 in PBS elicited an 8-fold increase in fluorescence intensity, suggesting superior environmental sensitivity. Fluorescence lifetime measurements also confirmed these results. Furthermore, compound 9 showed time- and concentration-dependent in vitro cellular uptake behavior in MCF-7 cells and other cancer cell lines. CLSM assay of tumor sections from MCF-7 nude mice also confirmed the possibility of tumor imaging.

KW - Cancer cell imaging

KW - Fluorescence

KW - Low molecular weight

KW - Molecular rotor

UR - https://www.scopus.com/pages/publications/85081720060

U2 - 10.1016/j.dyepig.2020.108353

DO - 10.1016/j.dyepig.2020.108353

M3 - 文章

AN - SCOPUS:85081720060

SN - 0143-7208

VL - 178

JO - Dyes and Pigments

JF - Dyes and Pigments

M1 - 108353

ER -

Synthesis and fluorescent studies of a low molecular weight rotor for living cancer cell imaging

Abstract

Keywords

UN SDGs

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