Synergistic effects of heterogeneous mature compost and aeration rate on humification and nitrogen fixing during kitchen waste composting

Sludge mature compost (SMC) is notable for its high production, easy accessibility, and stable supply. This study investigated the impact of the SMC addition and different aeration rates on the humification and nitrogen fixing process during kitchen waste composting. The results demonstrated that addition of SMC prolonged the thermophilic phase, as a comparison, increased aeration shortened this phase. The addition of SMC and increased aeration enhanced humus formation and nitrogen retention. SMC introduced more amide and polysaccharide compounds into the compost, promoting the Maillard humification pathway. Additionally, both SMC and high aeration inhibited denitrification: the SMC reduced the abundance of the nirK gene, while high aeration decreased the abundance of nosZ gene. Network analysis revealed that higher aeration enhanced fungal interactions but diminished bacterial interactions. Conversely, SMC addition bolstered both bacterial and fungal interactions. The final compost product with SMC addition showed a 11.56%–44.19% reduction in antibiotic resistance gene content compared with the control group, and heavy metal contents remained within safe application limits. The combination of high SMC addition and high aeration achieved optimal humification and nitrogen retention, underscoring its potential as a promising solution for kitchen waste composting.