TY - JOUR
T1 - Surface-enhanced Raman Scattering Technology Based on WO3 Film for Detection of VEGF
AU - Liu, Xiaoyan
AU - Zhou, Yan
AU - Zheng, Tingting
AU - Tian, Yang
N1 - Publisher Copyright:
© 2021, Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH.
PY - 2021/8
Y1 - 2021/8
N2 - With the advancement of nanomaterials for surface-enhanced Raman scattering(SERS) detection, a deeper understanding of the chemical mechanism(CM) and further applications has been achieved. Herein, we prepared a porous tungsten trioxide(WO3) film by the pulse electrodeposition method, and constructed a WO3 film SERS aptasensor. With methylene blue(MB) as the adsorption molecule, the developed WO3 film SERS aptasensor revealed remarkable Raman activity. Through experimental data and theoretical calculations, we found that the significant SERS enhancement[enhancement factor(EF)=1.5×106] was due to the CM based on charge transfer and molecular resonance. Utilizing the Raman response of MB on the WO3 film and specific aptamers, we successfully developed the aptamer sensor by covalently attaching the MB modified aptamer to the WO3 film. The sensor realized the specific and sensitive determination of vascular endothelial growth factor(VEGF) with the detection limit down to 8.7 pg/mL. In addition, the developed aptasensor indicated the excellent selectivity among other interferences, such as metal ions, reactive oxygen species(ROS), and proteins. This WO3 film SERS aptasensor not only contributed to the study of the enhancement mechanism of semiconductor material, but also provided a powerful platform for the sensitive detection of VEGF, possessing a great potential in the real-time monitoring of biomarkers of glioblastoma in vitro.
AB - With the advancement of nanomaterials for surface-enhanced Raman scattering(SERS) detection, a deeper understanding of the chemical mechanism(CM) and further applications has been achieved. Herein, we prepared a porous tungsten trioxide(WO3) film by the pulse electrodeposition method, and constructed a WO3 film SERS aptasensor. With methylene blue(MB) as the adsorption molecule, the developed WO3 film SERS aptasensor revealed remarkable Raman activity. Through experimental data and theoretical calculations, we found that the significant SERS enhancement[enhancement factor(EF)=1.5×106] was due to the CM based on charge transfer and molecular resonance. Utilizing the Raman response of MB on the WO3 film and specific aptamers, we successfully developed the aptamer sensor by covalently attaching the MB modified aptamer to the WO3 film. The sensor realized the specific and sensitive determination of vascular endothelial growth factor(VEGF) with the detection limit down to 8.7 pg/mL. In addition, the developed aptasensor indicated the excellent selectivity among other interferences, such as metal ions, reactive oxygen species(ROS), and proteins. This WO3 film SERS aptasensor not only contributed to the study of the enhancement mechanism of semiconductor material, but also provided a powerful platform for the sensitive detection of VEGF, possessing a great potential in the real-time monitoring of biomarkers of glioblastoma in vitro.
KW - Charge transfer
KW - Glioblastoma
KW - Surface-enhanced Raman scattering
KW - Vascular endothelial growth factor(VEGF)
KW - WO
UR - https://www.scopus.com/pages/publications/85108003743
U2 - 10.1007/s40242-021-1192-5
DO - 10.1007/s40242-021-1192-5
M3 - 文章
AN - SCOPUS:85108003743
SN - 1005-9040
VL - 37
SP - 900
EP - 905
JO - Chemical Research in Chinese Universities
JF - Chemical Research in Chinese Universities
IS - 4
ER -