Suppression subtractive hybridization cloning of cDNAs of differentially expressed genes in dovetree (Davidia involucrata) bracts

Yun Xiang Li; L. Chen; Lin Juan; Yufeng Li; Fang Chen

doi:10.1007/BF02782458

Suppression subtractive hybridization cloning of cDNAs of differentially expressed genes in dovetree (Davidia involucrata) bracts

Yun Xiang Li
, L. Chen
, Lin Juan
, Yufeng Li
, Fang Chen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

A subtracted cDNA library for Davidia involucrata was constructed using suppression subtractive hybridization (SSH). mRNA isolated from young leaves was used as a "driver," and mRNAs isolated from young bracts were used as "testers." The differentially expressed cDNA fragments in bracts were identified by differential screening. Of the 16 clones selected randomly from the screened library, 8 were known genes found in GenBank, and 2 had no similar sequences. Northern blot analysis revealed that the expression level of P1A5 cDNAs selected randomly was dominantly expressed in bracts. This indicates that SSH can be used to clone differentially expressed cDNAs in D. involucrata bracts.

Original language	English
Pages (from-to)	231-238
Number of pages	8
Journal	Plant Molecular Biology Reporter
Volume	20
Issue number	3
DOIs	https://doi.org/10.1007/BF02782458
State	Published - Sep 2002
Externally published	Yes

Keywords

Bract
Davidia involucrata
Leaf
Northern blot analysis
SSH

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10.1007/BF02782458

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title = "Suppression subtractive hybridization cloning of cDNAs of differentially expressed genes in dovetree (Davidia involucrata) bracts",

abstract = "A subtracted cDNA library for Davidia involucrata was constructed using suppression subtractive hybridization (SSH). mRNA isolated from young leaves was used as a {"}driver,{"} and mRNAs isolated from young bracts were used as {"}testers.{"} The differentially expressed cDNA fragments in bracts were identified by differential screening. Of the 16 clones selected randomly from the screened library, 8 were known genes found in GenBank, and 2 had no similar sequences. Northern blot analysis revealed that the expression level of P1A5 cDNAs selected randomly was dominantly expressed in bracts. This indicates that SSH can be used to clone differentially expressed cDNAs in D. involucrata bracts.",

keywords = "Bract, Davidia involucrata, Leaf, Northern blot analysis, SSH",

author = "Li, \{Yun Xiang\} and L. Chen and Lin Juan and Yufeng Li and Fang Chen",

year = "2002",

month = sep,

doi = "10.1007/BF02782458",

language = "英语",

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T1 - Suppression subtractive hybridization cloning of cDNAs of differentially expressed genes in dovetree (Davidia involucrata) bracts

AU - Li, Yun Xiang

AU - Chen, L.

AU - Juan, Lin

AU - Li, Yufeng

AU - Chen, Fang

PY - 2002/9

Y1 - 2002/9

N2 - A subtracted cDNA library for Davidia involucrata was constructed using suppression subtractive hybridization (SSH). mRNA isolated from young leaves was used as a "driver," and mRNAs isolated from young bracts were used as "testers." The differentially expressed cDNA fragments in bracts were identified by differential screening. Of the 16 clones selected randomly from the screened library, 8 were known genes found in GenBank, and 2 had no similar sequences. Northern blot analysis revealed that the expression level of P1A5 cDNAs selected randomly was dominantly expressed in bracts. This indicates that SSH can be used to clone differentially expressed cDNAs in D. involucrata bracts.

AB - A subtracted cDNA library for Davidia involucrata was constructed using suppression subtractive hybridization (SSH). mRNA isolated from young leaves was used as a "driver," and mRNAs isolated from young bracts were used as "testers." The differentially expressed cDNA fragments in bracts were identified by differential screening. Of the 16 clones selected randomly from the screened library, 8 were known genes found in GenBank, and 2 had no similar sequences. Northern blot analysis revealed that the expression level of P1A5 cDNAs selected randomly was dominantly expressed in bracts. This indicates that SSH can be used to clone differentially expressed cDNAs in D. involucrata bracts.

KW - Bract

KW - Davidia involucrata

KW - Leaf

KW - Northern blot analysis

KW - SSH

UR - https://www.scopus.com/pages/publications/4544248173

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DO - 10.1007/BF02782458

M3 - 文章

AN - SCOPUS:4544248173

SN - 0735-9640

VL - 20

SP - 231

EP - 238

JO - Plant Molecular Biology Reporter

JF - Plant Molecular Biology Reporter

IS - 3

ER -

Suppression subtractive hybridization cloning of cDNAs of differentially expressed genes in dovetree (Davidia involucrata) bracts

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Keywords

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