Suppression of sidelobes in single-photon 4pi confocal microscopy by förster resonant energy transfer

Jianfang Chen; Ya Cheng

doi:10.1142/S1793545812500162

Suppression of sidelobes in single-photon 4pi confocal microscopy by förster resonant energy transfer

Jianfang Chen, Ya Cheng

CAS - Shanghai Institute of Optics and Fine Mechanics

Research output: Contribution to journal › Article › peer-review

Abstract

Recently, we theoretically demonstrate that utilization of silica nanobeads co-doped with Cy3 and Cy5 molecules instead of single dye molecules as fluorescent labels can enable optical resolutions far beyond the diffraction-limit. Here, we show that by combining the 4Pi microscopy and the novel fluorescent label, it is possible to completely suppress the sidelobes in 4Pi focal spot and significantly enhance the optical resolution in the axial direction.

Original language	English
Article number	1250016
Journal	Journal of Innovative Optical Health Sciences
Volume	5
Issue number	3
DOIs	https://doi.org/10.1142/S1793545812500162
State	Published - Jul 2012
Externally published	Yes

Keywords

4Pi microscopy
Fluorescent dye
confocal
far-field imaging
super-resolution

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10.1142/S1793545812500162

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title = "Suppression of sidelobes in single-photon 4pi confocal microscopy by f{\"o}rster resonant energy transfer",

abstract = "Recently, we theoretically demonstrate that utilization of silica nanobeads co-doped with Cy3 and Cy5 molecules instead of single dye molecules as fluorescent labels can enable optical resolutions far beyond the diffraction-limit. Here, we show that by combining the 4Pi microscopy and the novel fluorescent label, it is possible to completely suppress the sidelobes in 4Pi focal spot and significantly enhance the optical resolution in the axial direction.",

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AU - Cheng, Ya

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AB - Recently, we theoretically demonstrate that utilization of silica nanobeads co-doped with Cy3 and Cy5 molecules instead of single dye molecules as fluorescent labels can enable optical resolutions far beyond the diffraction-limit. Here, we show that by combining the 4Pi microscopy and the novel fluorescent label, it is possible to completely suppress the sidelobes in 4Pi focal spot and significantly enhance the optical resolution in the axial direction.

KW - 4Pi microscopy

KW - Fluorescent dye

KW - confocal

KW - far-field imaging

KW - super-resolution

UR - https://www.scopus.com/pages/publications/84865472473

U2 - 10.1142/S1793545812500162

DO - 10.1142/S1793545812500162

M3 - 文章

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JO - Journal of Innovative Optical Health Sciences

JF - Journal of Innovative Optical Health Sciences

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ER -

Suppression of sidelobes in single-photon 4pi confocal microscopy by förster resonant energy transfer

Abstract

Keywords

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