Suppression of endogenous gene silencing by bidirectional cytoplasmic RNA decay in Arabidopsis

Xinyan Zhang; Ying Zhu; Xiaodan Liu; Xinyu Hong; Yang Xu; Ping Zhu; Yang Shen; Huihui Wu; Yusi Ji; Xing Wen; Chen Zhang; Qiong Zhao; Yichuan Wang; Jian Lu; Hongwei Guo

doi:10.1126/science.aaa2618

Suppression of endogenous gene silencing by bidirectional cytoplasmic RNA decay in Arabidopsis

Xinyan Zhang, Ying Zhu, Xiaodan Liu, Xinyu Hong, Yang Xu, Ping Zhu, Yang Shen, Huihui Wu, Yusi Ji, Xing Wen, Chen Zhang, Qiong Zhao, Yichuan Wang, Jian Lu, Hongwei Guo

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147 Scopus citations

Abstract

Plant immunity against foreign gene invasion takes advantage of posttranscriptional gene silencing (PTGS). How plants elaborately avert inappropriate PTGS of endogenous coding genes remains unclear. We demonstrate in Arabidopsis that both 5'-3' and 3'-5' cytoplasmic RNA decay pathways act as repressors of transgene and endogenous PTGS. Disruption of bidirectional cytoplasmic RNA decay leads to pleiotropic developmental defects and drastic transcriptomic alterations, which are substantially rescued by PTGS mutants. Upon dysfunction of bidirectional RNA decay, a large number of 21-to 22-nucleotide endogenous small interfering RNAs are produced from coding transcripts, including multiple microRNA targets, which could interfere with their cognate gene expression and functions. This study highlights the risk of unwanted PTGS and identifies cytoplasmic RNA decay pathways as safeguards of plant transcriptome and development.

Original language	English
Pages (from-to)	120-123
Number of pages	4
Journal	Science
Volume	348
Issue number	6230
DOIs	https://doi.org/10.1126/science.aaa2618
State	Published - 3 Apr 2015
Externally published	Yes

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title = "Suppression of endogenous gene silencing by bidirectional cytoplasmic RNA decay in Arabidopsis",

abstract = "Plant immunity against foreign gene invasion takes advantage of posttranscriptional gene silencing (PTGS). How plants elaborately avert inappropriate PTGS of endogenous coding genes remains unclear. We demonstrate in Arabidopsis that both 5'-3' and 3'-5' cytoplasmic RNA decay pathways act as repressors of transgene and endogenous PTGS. Disruption of bidirectional cytoplasmic RNA decay leads to pleiotropic developmental defects and drastic transcriptomic alterations, which are substantially rescued by PTGS mutants. Upon dysfunction of bidirectional RNA decay, a large number of 21-to 22-nucleotide endogenous small interfering RNAs are produced from coding transcripts, including multiple microRNA targets, which could interfere with their cognate gene expression and functions. This study highlights the risk of unwanted PTGS and identifies cytoplasmic RNA decay pathways as safeguards of plant transcriptome and development.",

author = "Xinyan Zhang and Ying Zhu and Xiaodan Liu and Xinyu Hong and Yang Xu and Ping Zhu and Yang Shen and Huihui Wu and Yusi Ji and Xing Wen and Chen Zhang and Qiong Zhao and Yichuan Wang and Jian Lu and Hongwei Guo",

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doi = "10.1126/science.aaa2618",

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TY - JOUR

T1 - Suppression of endogenous gene silencing by bidirectional cytoplasmic RNA decay in Arabidopsis

AU - Zhang, Xinyan

AU - Zhu, Ying

AU - Liu, Xiaodan

AU - Hong, Xinyu

AU - Xu, Yang

AU - Zhu, Ping

AU - Shen, Yang

AU - Wu, Huihui

AU - Ji, Yusi

AU - Wen, Xing

AU - Zhang, Chen

AU - Zhao, Qiong

AU - Wang, Yichuan

AU - Lu, Jian

AU - Guo, Hongwei

PY - 2015/4/3

Y1 - 2015/4/3

N2 - Plant immunity against foreign gene invasion takes advantage of posttranscriptional gene silencing (PTGS). How plants elaborately avert inappropriate PTGS of endogenous coding genes remains unclear. We demonstrate in Arabidopsis that both 5'-3' and 3'-5' cytoplasmic RNA decay pathways act as repressors of transgene and endogenous PTGS. Disruption of bidirectional cytoplasmic RNA decay leads to pleiotropic developmental defects and drastic transcriptomic alterations, which are substantially rescued by PTGS mutants. Upon dysfunction of bidirectional RNA decay, a large number of 21-to 22-nucleotide endogenous small interfering RNAs are produced from coding transcripts, including multiple microRNA targets, which could interfere with their cognate gene expression and functions. This study highlights the risk of unwanted PTGS and identifies cytoplasmic RNA decay pathways as safeguards of plant transcriptome and development.

AB - Plant immunity against foreign gene invasion takes advantage of posttranscriptional gene silencing (PTGS). How plants elaborately avert inappropriate PTGS of endogenous coding genes remains unclear. We demonstrate in Arabidopsis that both 5'-3' and 3'-5' cytoplasmic RNA decay pathways act as repressors of transgene and endogenous PTGS. Disruption of bidirectional cytoplasmic RNA decay leads to pleiotropic developmental defects and drastic transcriptomic alterations, which are substantially rescued by PTGS mutants. Upon dysfunction of bidirectional RNA decay, a large number of 21-to 22-nucleotide endogenous small interfering RNAs are produced from coding transcripts, including multiple microRNA targets, which could interfere with their cognate gene expression and functions. This study highlights the risk of unwanted PTGS and identifies cytoplasmic RNA decay pathways as safeguards of plant transcriptome and development.

UR - https://www.scopus.com/pages/publications/84928755185

U2 - 10.1126/science.aaa2618

DO - 10.1126/science.aaa2618

M3 - 文章

C2 - 25838384

AN - SCOPUS:84928755185

SN - 0036-8075

VL - 348

SP - 120

EP - 123

JO - Science

JF - Science

IS - 6230

ER -

Suppression of endogenous gene silencing by bidirectional cytoplasmic RNA decay in Arabidopsis

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