TY - JOUR
T1 - Super-Resolution Monitoring of Mitochondrial Dynamics upon Time-Gated Photo-Triggered Release of Nitric Oxide
AU - He, Haihong
AU - Ye, Zhiwei
AU - Xiao, Yi
AU - Yang, Wei
AU - Qian, Xuhong
AU - Yang, Youjun
N1 - Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/2/6
Y1 - 2018/2/6
N2 - Nitric oxide (NO) potentially plays a regulatory role in mitochondrial fusion and fission, which are vital to cell survival and implicated in health, disease, and aging. Molecular tools facilitating the study of the relationship between NO and mitochondrial dynamics are in need. We have recently developed a novel NO donor (NOD550). Upon photoactivation, NOD550 decomposes to release two NO molecules and a fluorophore. The NO release could be spatially mapped with subdiffraction resolution and with a temporal resolution of 10 s. Due to the preferential localization of NOD550 at mitochondria, morphology and dynamics of mitochondria could be monitored upon NO release from NOD550.
AB - Nitric oxide (NO) potentially plays a regulatory role in mitochondrial fusion and fission, which are vital to cell survival and implicated in health, disease, and aging. Molecular tools facilitating the study of the relationship between NO and mitochondrial dynamics are in need. We have recently developed a novel NO donor (NOD550). Upon photoactivation, NOD550 decomposes to release two NO molecules and a fluorophore. The NO release could be spatially mapped with subdiffraction resolution and with a temporal resolution of 10 s. Due to the preferential localization of NOD550 at mitochondria, morphology and dynamics of mitochondria could be monitored upon NO release from NOD550.
UR - https://www.scopus.com/pages/publications/85041386289
U2 - 10.1021/acs.analchem.7b04510
DO - 10.1021/acs.analchem.7b04510
M3 - 文章
C2 - 29316789
AN - SCOPUS:85041386289
SN - 0003-2700
VL - 90
SP - 2164
EP - 2169
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 3
ER -