Abstract
AIM: To detect the immunogenicity of HIV-1 gag DNA vaccine. METHODS: The serum antibody titers of the immunized mice were detected by ELISA. The numbers of spleen's CD4(+) and CD8(+) T cells were analyzed by the fluorescent antibody staining and the cytotoxic activity was determined by lactate dehydrogenase (LDH) release assay. RESULTS: The titers of the serum antibodies and the numbers and cytotoxic activity of spleen's T lymphocyte subsets had significant differences (P<0.05 and P<0.01, respectively) between pVAXGAG and pVAX1 vaccinated groups. CONCLUSION: HIV-1 DNA vaccine plasmid pVAXGAG could elicit both the specific humoral and cellular immunities in BALB/c mice.
| Original language | English |
|---|---|
| Pages (from-to) | 272-273 |
| Number of pages | 2 |
| Journal | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology |
| Volume | 20 |
| Issue number | 3 |
| State | Published - May 2004 |
| Externally published | Yes |
