Studies on chemical constituents of cytotoxic fraction from leaves of Elaeagnus pungens

Objective: To study the chemical constituents possessing cytotoxicity activity from Elaeagnus pungens. Method: The constituents were separated through repeated Chromatographic methods and their structures were elucidated by spectral analysis. Result: Five compounds were isolated from the ethyl acetate ether extract of leaves of E. pungens. Their structures were elucidated as 4-hydroxybenzoic acid (1), 3, 3′-dimethoxyquercetin (2), caffeic acid methyl ester (3), methyl 3, 4-dihydroxybenzoate (4), spingic acid (5), 4-methoxylbenzoic acid (6), 3-methylkaempferol (7), kaempferol-3-O-β-D- glucoside (8), dausosterol (9). Conclusion: Compounds 1-8 were isolated from this plant for the first time.