Stability and activity of chymotrypsie immobilized on magnetic nanogels covered with carboxyl groups

a-chymotrypsin as model enzyme was anchored to the carboxyl-functionalized magnetic nanogels prepared by in situ photochemical polymerization. Furthermore, to explore the optimum immobilization, the effects of immobilization time, pH of the reaction mixture, and proportion of enzyme to the magnetic nanogels were studied. The immobilized enzyme was stable in the presence of enzyme denaturation surfactants, and maintained their activity against protein-digesting enzyme. The immobilized enzyme exhibited hydrolysis activity against the substrate of casein, and cleaved the substrate into small fragments.