TY - JOUR
T1 - Single-atom aptamer anchoring enables light-addressable multiplexed biosensing for early detection of pancreatic cancer
AU - Li, Dan
AU - Qian, Jianfeng
AU - Li, Yanrong
AU - Liu, Yibin
AU - Xu, Xiankui
AU - Zhou, Xue
AU - Zhang, Zhonghai
AU - Meng, Jun
AU - Qin, Kai
N1 - Publisher Copyright:
© 2025 Elsevier B.V.
PY - 2025/11/15
Y1 - 2025/11/15
N2 - Pancreatic cancer remains one of the deadliest malignancies due to its silent progression and lack of early diagnostic tools. Here, we report a light-addressable photoelectrochemical (LAMT-PEC) biosensing system featuring single-atom Au1-TiO2 photoelectrodes for the simultaneous detection of five pancreatic cancer biomarkers in blood: CA19-9, CEA, AFP, CA125, and CA724. By anchoring aptamers at atomic precision, the platform achieves sub-pg/mL sensitivity, minimizes nonspecific binding, and demonstrates high anti-interference capability. When coupled with a machine learning algorithm, the system enables precise discrimination of early-stage pancreatic cancer from healthy individuals with an AUC of 97.4 %, significantly outperforming CA19-9 alone. The LAMT-PEC platform presents a scalable, high-throughput, and clinically relevant strategy for early cancer screening, with broad applicability to multiplex diagnostics.
AB - Pancreatic cancer remains one of the deadliest malignancies due to its silent progression and lack of early diagnostic tools. Here, we report a light-addressable photoelectrochemical (LAMT-PEC) biosensing system featuring single-atom Au1-TiO2 photoelectrodes for the simultaneous detection of five pancreatic cancer biomarkers in blood: CA19-9, CEA, AFP, CA125, and CA724. By anchoring aptamers at atomic precision, the platform achieves sub-pg/mL sensitivity, minimizes nonspecific binding, and demonstrates high anti-interference capability. When coupled with a machine learning algorithm, the system enables precise discrimination of early-stage pancreatic cancer from healthy individuals with an AUC of 97.4 %, significantly outperforming CA19-9 alone. The LAMT-PEC platform presents a scalable, high-throughput, and clinically relevant strategy for early cancer screening, with broad applicability to multiplex diagnostics.
KW - Light-addressable detection
KW - Machine learning
KW - Multiplex biomarker
KW - Pancreatic cancer
KW - Photoelectrochemical biosensor
KW - Single-atom Au
UR - https://www.scopus.com/pages/publications/105010872845
U2 - 10.1016/j.bios.2025.117792
DO - 10.1016/j.bios.2025.117792
M3 - 文章
AN - SCOPUS:105010872845
SN - 0956-5663
VL - 288
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
M1 - 117792
ER -
