Simultaneous determination of flavonoids and anthraquinones in chrysanthemum by capillary electrophoresis with amperometry detection

Yin Yan Zhang; Zi Cheng Li; Jin Kun Zhu; Zhi Yong Yang; Qing Jiang Wang; Pin Gang He; Yu Zhi Fang

doi:10.1016/j.cclet.2010.04.040

Simultaneous determination of flavonoids and anthraquinones in chrysanthemum by capillary electrophoresis with amperometry detection

Yin Yan Zhang, Zi Cheng Li, Jin Kun Zhu, Zhi Yong Yang, Qing Jiang Wang, Pin Gang He, Yu Zhi Fang

School of Chemistry and Molecular Engineering

East China Normal University

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

A high-performance capillary electrophoresis with amperometry detection method (CE-AD) has been developed for the analysis of flavonoids and anthraquinones (emodin, kaempferol, apigenin, luteolin and rhein) in chrysanthemum. Under optimum conditions, these five analytes were base-line separated within 17min using a borate-phosphate running buffer (1.5×10^-2mol/L borate-3×10^-2mol/L phosphate running buffer, pH 9.0) at a working potential of +0.90V (vs. SCE) and a separation voltage of 19kV. The linear relationship between concentration and current response was obtained with detection limits (S/N=3) ranging from 1.0×10^-7 to 2.1×10^-7g/mL for all analytes. This proposed method was successfully used in the analysis of four kinds of chrysanthemum with relatively simple extraction procedures, the assay results were satisfactory.

Original language	English
Pages (from-to)	1231-1234
Number of pages	4
Journal	Chinese Chemical Letters
Volume	21
Issue number	10
DOIs	https://doi.org/10.1016/j.cclet.2010.04.040
State	Published - Oct 2010

Keywords

Amperometry detection
Anthraquinones
Capillary electrophoresis
Chrysanthemu
Flavonoids

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10.1016/j.cclet.2010.04.040

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@article{b22139cb51a54008959c336336fa7e5c,

title = "Simultaneous determination of flavonoids and anthraquinones in chrysanthemum by capillary electrophoresis with amperometry detection",

abstract = "A high-performance capillary electrophoresis with amperometry detection method (CE-AD) has been developed for the analysis of flavonoids and anthraquinones (emodin, kaempferol, apigenin, luteolin and rhein) in chrysanthemum. Under optimum conditions, these five analytes were base-line separated within 17min using a borate-phosphate running buffer (1.5×10-2mol/L borate-3×10-2mol/L phosphate running buffer, pH 9.0) at a working potential of +0.90V (vs. SCE) and a separation voltage of 19kV. The linear relationship between concentration and current response was obtained with detection limits (S/N=3) ranging from 1.0×10-7 to 2.1×10-7g/mL for all analytes. This proposed method was successfully used in the analysis of four kinds of chrysanthemum with relatively simple extraction procedures, the assay results were satisfactory.",

keywords = "Amperometry detection, Anthraquinones, Capillary electrophoresis, Chrysanthemu, Flavonoids",

author = "Zhang, \{Yin Yan\} and Li, \{Zi Cheng\} and Zhu, \{Jin Kun\} and Yang, \{Zhi Yong\} and Wang, \{Qing Jiang\} and He, \{Pin Gang\} and Fang, \{Yu Zhi\}",

year = "2010",

month = oct,

doi = "10.1016/j.cclet.2010.04.040",

language = "英语",

volume = "21",

pages = "1231--1234",

journal = "Chinese Chemical Letters",

issn = "1001-8417",

publisher = "Elsevier B.V.",

number = "10",

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TY - JOUR

T1 - Simultaneous determination of flavonoids and anthraquinones in chrysanthemum by capillary electrophoresis with amperometry detection

AU - Zhang, Yin Yan

AU - Li, Zi Cheng

AU - Zhu, Jin Kun

AU - Yang, Zhi Yong

AU - Wang, Qing Jiang

AU - He, Pin Gang

AU - Fang, Yu Zhi

PY - 2010/10

Y1 - 2010/10

N2 - A high-performance capillary electrophoresis with amperometry detection method (CE-AD) has been developed for the analysis of flavonoids and anthraquinones (emodin, kaempferol, apigenin, luteolin and rhein) in chrysanthemum. Under optimum conditions, these five analytes were base-line separated within 17min using a borate-phosphate running buffer (1.5×10-2mol/L borate-3×10-2mol/L phosphate running buffer, pH 9.0) at a working potential of +0.90V (vs. SCE) and a separation voltage of 19kV. The linear relationship between concentration and current response was obtained with detection limits (S/N=3) ranging from 1.0×10-7 to 2.1×10-7g/mL for all analytes. This proposed method was successfully used in the analysis of four kinds of chrysanthemum with relatively simple extraction procedures, the assay results were satisfactory.

AB - A high-performance capillary electrophoresis with amperometry detection method (CE-AD) has been developed for the analysis of flavonoids and anthraquinones (emodin, kaempferol, apigenin, luteolin and rhein) in chrysanthemum. Under optimum conditions, these five analytes were base-line separated within 17min using a borate-phosphate running buffer (1.5×10-2mol/L borate-3×10-2mol/L phosphate running buffer, pH 9.0) at a working potential of +0.90V (vs. SCE) and a separation voltage of 19kV. The linear relationship between concentration and current response was obtained with detection limits (S/N=3) ranging from 1.0×10-7 to 2.1×10-7g/mL for all analytes. This proposed method was successfully used in the analysis of four kinds of chrysanthemum with relatively simple extraction procedures, the assay results were satisfactory.

KW - Amperometry detection

KW - Anthraquinones

KW - Capillary electrophoresis

KW - Chrysanthemu

KW - Flavonoids

UR - https://www.scopus.com/pages/publications/77955845831

U2 - 10.1016/j.cclet.2010.04.040

DO - 10.1016/j.cclet.2010.04.040

M3 - 文章

AN - SCOPUS:77955845831

SN - 1001-8417

VL - 21

SP - 1231

EP - 1234

JO - Chinese Chemical Letters

JF - Chinese Chemical Letters

IS - 10

ER -

Simultaneous determination of flavonoids and anthraquinones in chrysanthemum by capillary electrophoresis with amperometry detection

Abstract

Keywords

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