Abstract
The fabrication and application of a novel electrochemical detection (ED) system with a poly(bromophenol blue) (PBPB) film chemically modified electrode (CME) for high performance liquid chromatography (HPLC) were described. The electrochemical behaviors of 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) at this CME were investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). It was found that the PBPB CME efficiently exhibited electrocatalytic effect on the current responses of 5-HT and 5-HIAA with relatively high sensitivity, stability and long life of activity. In HPLC-ED, the two analytes had good and stable current responses at the CME and their linear ranges were over four orders of magnitude (R ≥ 0.9992) with the detection limits being 0.25 nmol L-1 for 5-HT and 0.50 nmol L-1 for 5-HIAA. The application of this method for the determination of 5-HT and 5-HIAA in urine samples from patients with acute appendicitis (AA) was satisfactory.
| Original language | English |
|---|---|
| Pages (from-to) | 14-19 |
| Number of pages | 6 |
| Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
| Volume | 846 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - 1 Feb 2007 |
Keywords
- 5-Hydroxyindoleacetic acid
- 5-Hydroxytryptamine
- Acute appendicitis
- HPLC-ED
- Poly(bromophenol blue) film