Simultaneous Detection of Intracellular Nitric Oxide and Peroxynitrite by a Surface-Enhanced Raman Scattering Nanosensor with Dual Reactivity

Hua Ying Chen; Essy Kouadio Fodjo; Lei Jiang; Shuai Chang; Jia Bin Li; De Sheng Zhan; Hai Xin Gu; Da Wei Li

doi:10.1021/acssensors.9b01740

Simultaneous Detection of Intracellular Nitric Oxide and Peroxynitrite by a Surface-Enhanced Raman Scattering Nanosensor with Dual Reactivity

Hua Ying Chen
, Essy Kouadio Fodjo
, Lei Jiang
, Shuai Chang
, Jia Bin Li
, De Sheng Zhan
, Hai Xin Gu
, Da Wei Li^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

A functional surface-enhanced Raman scattering (SERS) nanosensor which can simultaneously detect nitric oxide (NO) and peroxynitrite (ONOO^-) in living cells is explored. The SERS nanosensor is fabricated through modifying gold nanoparticles (AuNPs) with newly synthesized 3,4-diaminophenylboronic acid pinacol ester (DAPBAP), which has two reactive groups. The simultaneous detection achieved in this work is not only because of the SERS spectral changes of the nanosensor resulting from the dual reactivity of DAPBAP on AuNPs with NO and ONOO^- but also by the narrow SERS bands suitable for multiplex detection. Owing to the combination of SERS fingerprinting information and chemical reaction specificity, the nanosensor has great selectivity for NO and ONOO^-, respectively. In addition, the nanosensor has a wide linearity range from 0 to 1.0 × 10^-4 M with a submicromolar sensitivity. More importantly, simultaneous monitoring of NO and ONOO^- in the Raw264.7

Original language	English
Pages (from-to)	3234-3239
Number of pages	6
Journal	ACS Sensors
Volume	4
Issue number	12
DOIs	https://doi.org/10.1021/acssensors.9b01740
State	Published - 27 Dec 2019
Externally published	Yes

Keywords

SERS
macrophages
nanosensor
nitric oxide
peroxynitrite
simultaneous monitoring

Access to Document

10.1021/acssensors.9b01740

Cite this

@article{a2f467b206954e138c0dd861ac4f47b6,

title = "Simultaneous Detection of Intracellular Nitric Oxide and Peroxynitrite by a Surface-Enhanced Raman Scattering Nanosensor with Dual Reactivity",

abstract = "A functional surface-enhanced Raman scattering (SERS) nanosensor which can simultaneously detect nitric oxide (NO) and peroxynitrite (ONOO-) in living cells is explored. The SERS nanosensor is fabricated through modifying gold nanoparticles (AuNPs) with newly synthesized 3,4-diaminophenylboronic acid pinacol ester (DAPBAP), which has two reactive groups. The simultaneous detection achieved in this work is not only because of the SERS spectral changes of the nanosensor resulting from the dual reactivity of DAPBAP on AuNPs with NO and ONOO- but also by the narrow SERS bands suitable for multiplex detection. Owing to the combination of SERS fingerprinting information and chemical reaction specificity, the nanosensor has great selectivity for NO and ONOO-, respectively. In addition, the nanosensor has a wide linearity range from 0 to 1.0 × 10-4 M with a submicromolar sensitivity. More importantly, simultaneous monitoring of NO and ONOO- in the Raw264.7",

keywords = "SERS, macrophages, nanosensor, nitric oxide, peroxynitrite, simultaneous monitoring",

author = "Chen, \{Hua Ying\} and \{Kouadio Fodjo\}, Essy and Lei Jiang and Shuai Chang and Li, \{Jia Bin\} and Zhan, \{De Sheng\} and Gu, \{Hai Xin\} and Li, \{Da Wei\}",

note = "Publisher Copyright: {\textcopyright} 2019 American Chemical Society.",

year = "2019",

month = dec,

day = "27",

doi = "10.1021/acssensors.9b01740",

language = "英语",

volume = "4",

pages = "3234--3239",

journal = "ACS Sensors",

issn = "2379-3694",

publisher = "American Chemical Society",

number = "12",

}

TY - JOUR

T1 - Simultaneous Detection of Intracellular Nitric Oxide and Peroxynitrite by a Surface-Enhanced Raman Scattering Nanosensor with Dual Reactivity

AU - Chen, Hua Ying

AU - Kouadio Fodjo, Essy

AU - Jiang, Lei

AU - Chang, Shuai

AU - Li, Jia Bin

AU - Zhan, De Sheng

AU - Gu, Hai Xin

AU - Li, Da Wei

PY - 2019/12/27

Y1 - 2019/12/27

N2 - A functional surface-enhanced Raman scattering (SERS) nanosensor which can simultaneously detect nitric oxide (NO) and peroxynitrite (ONOO-) in living cells is explored. The SERS nanosensor is fabricated through modifying gold nanoparticles (AuNPs) with newly synthesized 3,4-diaminophenylboronic acid pinacol ester (DAPBAP), which has two reactive groups. The simultaneous detection achieved in this work is not only because of the SERS spectral changes of the nanosensor resulting from the dual reactivity of DAPBAP on AuNPs with NO and ONOO- but also by the narrow SERS bands suitable for multiplex detection. Owing to the combination of SERS fingerprinting information and chemical reaction specificity, the nanosensor has great selectivity for NO and ONOO-, respectively. In addition, the nanosensor has a wide linearity range from 0 to 1.0 × 10-4 M with a submicromolar sensitivity. More importantly, simultaneous monitoring of NO and ONOO- in the Raw264.7

AB - A functional surface-enhanced Raman scattering (SERS) nanosensor which can simultaneously detect nitric oxide (NO) and peroxynitrite (ONOO-) in living cells is explored. The SERS nanosensor is fabricated through modifying gold nanoparticles (AuNPs) with newly synthesized 3,4-diaminophenylboronic acid pinacol ester (DAPBAP), which has two reactive groups. The simultaneous detection achieved in this work is not only because of the SERS spectral changes of the nanosensor resulting from the dual reactivity of DAPBAP on AuNPs with NO and ONOO- but also by the narrow SERS bands suitable for multiplex detection. Owing to the combination of SERS fingerprinting information and chemical reaction specificity, the nanosensor has great selectivity for NO and ONOO-, respectively. In addition, the nanosensor has a wide linearity range from 0 to 1.0 × 10-4 M with a submicromolar sensitivity. More importantly, simultaneous monitoring of NO and ONOO- in the Raw264.7

KW - SERS

KW - macrophages

KW - nanosensor

KW - nitric oxide

KW - peroxynitrite

KW - simultaneous monitoring

UR - https://www.scopus.com/pages/publications/85076312333

U2 - 10.1021/acssensors.9b01740

DO - 10.1021/acssensors.9b01740

M3 - 文章

C2 - 31736302

AN - SCOPUS:85076312333

SN - 2379-3694

VL - 4

SP - 3234

EP - 3239

JO - ACS Sensors

JF - ACS Sensors

IS - 12

ER -

Simultaneous Detection of Intracellular Nitric Oxide and Peroxynitrite by a Surface-Enhanced Raman Scattering Nanosensor with Dual Reactivity

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this