Silencing of E6/E7 expression in cervical cancer stem-like cells

Wenyi Gu; Nigel McMillan; Chengzhong Yu

doi:10.1007/978-1-4939-2013-6_12

Silencing of E6/E7 expression in cervical cancer stem-like cells

Wenyi Gu, Nigel McMillan, Chengzhong Yu

School of Chemistry and Molecular Engineering

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Accumulating evidence supports the concept that cancer stem cells (CSCs) are responsible for the tumor recurrence and metastasis, the two major causes of cancer-related death. Therefore, CSC-targeted cancer therapy is important for the future development of more effective and advanced cancer therapy. One of the approaches is to specifically silence oncogene expression in CSCs and inhibit their growth. The significance of this approach is its specificity and ability to avoid multi-drug resistance of CSCs. In this chapter, we will describe a method of silencing HPV oncogenes E6/E7 in human cervical CSCs using HeLa cells as a model system.

Original language	English
Pages (from-to)	173-182
Number of pages	10
Journal	Methods in Molecular Biology
Volume	1249
DOIs	https://doi.org/10.1007/978-1-4939-2013-6_12
State	Published - 2015

Keywords

Cancer stem cells
Gene silence
HPV E6/E7
RNAi

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-2013-6_12

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title = "Silencing of E6/E7 expression in cervical cancer stem-like cells",

abstract = "Accumulating evidence supports the concept that cancer stem cells (CSCs) are responsible for the tumor recurrence and metastasis, the two major causes of cancer-related death. Therefore, CSC-targeted cancer therapy is important for the future development of more effective and advanced cancer therapy. One of the approaches is to specifically silence oncogene expression in CSCs and inhibit their growth. The significance of this approach is its specificity and ability to avoid multi-drug resistance of CSCs. In this chapter, we will describe a method of silencing HPV oncogenes E6/E7 in human cervical CSCs using HeLa cells as a model system.",

keywords = "Cancer stem cells, Gene silence, HPV E6/E7, RNAi",

author = "Wenyi Gu and Nigel McMillan and Chengzhong Yu",

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T1 - Silencing of E6/E7 expression in cervical cancer stem-like cells

AU - Gu, Wenyi

AU - McMillan, Nigel

AU - Yu, Chengzhong

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015.

PY - 2015

Y1 - 2015

N2 - Accumulating evidence supports the concept that cancer stem cells (CSCs) are responsible for the tumor recurrence and metastasis, the two major causes of cancer-related death. Therefore, CSC-targeted cancer therapy is important for the future development of more effective and advanced cancer therapy. One of the approaches is to specifically silence oncogene expression in CSCs and inhibit their growth. The significance of this approach is its specificity and ability to avoid multi-drug resistance of CSCs. In this chapter, we will describe a method of silencing HPV oncogenes E6/E7 in human cervical CSCs using HeLa cells as a model system.

AB - Accumulating evidence supports the concept that cancer stem cells (CSCs) are responsible for the tumor recurrence and metastasis, the two major causes of cancer-related death. Therefore, CSC-targeted cancer therapy is important for the future development of more effective and advanced cancer therapy. One of the approaches is to specifically silence oncogene expression in CSCs and inhibit their growth. The significance of this approach is its specificity and ability to avoid multi-drug resistance of CSCs. In this chapter, we will describe a method of silencing HPV oncogenes E6/E7 in human cervical CSCs using HeLa cells as a model system.

KW - Cancer stem cells

KW - Gene silence

KW - HPV E6/E7

KW - RNAi

UR - https://www.scopus.com/pages/publications/84958681099

U2 - 10.1007/978-1-4939-2013-6_12

DO - 10.1007/978-1-4939-2013-6_12

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AN - SCOPUS:84958681099

SN - 1064-3745

VL - 1249

SP - 173

EP - 182

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Silencing of E6/E7 expression in cervical cancer stem-like cells

Abstract

Keywords

UN SDGs

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