Signal-off and signal-on design for a label-free aptasensor based on target-induced self-assembly and abasic-site-binding ligands

Zhiai Xu; Yusuke Sato; Seiichi Nishizawa; Norio Teramae

doi:10.1002/chem.200901226

Signal-off and signal-on design for a label-free aptasensor based on target-induced self-assembly and abasic-site-binding ligands

Zhiai Xu
, Yusuke Sato
, Seiichi Nishizawa
, Norio Teramae^*

^*Corresponding author for this work

Tohoku University

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Getting an "A": A label-free adenosine aptasensor has been designed based on adenosine-induced selfassembly of aptamer fragments. One aptamer fragment contains an abasic (AP) site, and the AP-site-binding ligands (*) are used as fluorescence probes for signal-off and signal-on responses. The aptasensor can detect adenosine with high selectivity over the other three nucleotides.

Original language	English
Pages (from-to)	10375-10378
Number of pages	4
Journal	Chemistry - A European Journal
Volume	15
Issue number	40
DOIs	https://doi.org/10.1002/chem.200901226
State	Published - 12 Oct 2009
Externally published	Yes

Keywords

Abasic sites
Aptamers
Fluorescent probes
Nucleobases
Self-assembly

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10.1002/chem.200901226

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author = "Zhiai Xu and Yusuke Sato and Seiichi Nishizawa and Norio Teramae",

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AU - Nishizawa, Seiichi

AU - Teramae, Norio

PY - 2009/10/12

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N2 - Getting an "A": A label-free adenosine aptasensor has been designed based on adenosine-induced selfassembly of aptamer fragments. One aptamer fragment contains an abasic (AP) site, and the AP-site-binding ligands (*) are used as fluorescence probes for signal-off and signal-on responses. The aptasensor can detect adenosine with high selectivity over the other three nucleotides.

AB - Getting an "A": A label-free adenosine aptasensor has been designed based on adenosine-induced selfassembly of aptamer fragments. One aptamer fragment contains an abasic (AP) site, and the AP-site-binding ligands (*) are used as fluorescence probes for signal-off and signal-on responses. The aptasensor can detect adenosine with high selectivity over the other three nucleotides.

KW - Abasic sites

KW - Aptamers

KW - Fluorescent probes

KW - Nucleobases

KW - Self-assembly

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DO - 10.1002/chem.200901226

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ER -

Signal-off and signal-on design for a label-free aptasensor based on target-induced self-assembly and abasic-site-binding ligands

Abstract

Keywords

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