Sex identification based on AMEL gene PCR amplification from blue sheep (Pseudois nayaur) fecal DNA samples

X. Liu; Y. Y. Yang; X. M. Wang; Z. S. Liu; Z. H. Wang; Y. Z. Ding

doi:10.4238/2015.August.7.13

Sex identification based on AMEL gene PCR amplification from blue sheep (Pseudois nayaur) fecal DNA samples

X. Liu
, Y. Y. Yang
, X. M. Wang
, Z. S. Liu
, Z. H. Wang
, Y. Z. Ding^*

^*Corresponding author for this work

Shanghai International School of Chief Technology Officer

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The use of noninvasive genetic sampling to identify the sex of wild animals is an extremely valuable and important tool in molecular ecology and wildlife conservation. Sex determination using the amelogenin gene has been conducted in many species because only a single pair of primers is required to amplify both X- and Y-linked alleles. However, this method has not been used in field research with the feces of wildlife. In this study, we applied this method to 222 fecal samples from wild blue sheep (Pseudois nayaur) using amelogenin primers (SE47/SE48) after testing the effectiveness of sex determination using tissue samples and fecal samples from blue sheep of known sex. We found this method to be highly reliable (80.2%) for blue sheep. Amelogenin can be used to identify the sex of wild animals using fecal samples.

Original language	English
Pages (from-to)	9045-9052
Number of pages	8
Journal	Genetics and Molecular Research
Volume	14
Issue number	3
DOIs	https://doi.org/10.4238/2015.August.7.13
State	Published - 7 Aug 2015

Keywords

Amelogenin
Blue sheep
Fecal sample
Non-invasive genetic sample
Polymerase chain reaction-based sex-typing

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title = "Sex identification based on AMEL gene PCR amplification from blue sheep (Pseudois nayaur) fecal DNA samples",

abstract = "The use of noninvasive genetic sampling to identify the sex of wild animals is an extremely valuable and important tool in molecular ecology and wildlife conservation. Sex determination using the amelogenin gene has been conducted in many species because only a single pair of primers is required to amplify both X- and Y-linked alleles. However, this method has not been used in field research with the feces of wildlife. In this study, we applied this method to 222 fecal samples from wild blue sheep (Pseudois nayaur) using amelogenin primers (SE47/SE48) after testing the effectiveness of sex determination using tissue samples and fecal samples from blue sheep of known sex. We found this method to be highly reliable (80.2\%) for blue sheep. Amelogenin can be used to identify the sex of wild animals using fecal samples.",

keywords = "Amelogenin, Blue sheep, Fecal sample, Non-invasive genetic sample, Polymerase chain reaction-based sex-typing",

author = "X. Liu and Yang, \{Y. Y.\} and Wang, \{X. M.\} and Liu, \{Z. S.\} and Wang, \{Z. H.\} and Ding, \{Y. Z.\}",

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year = "2015",

month = aug,

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doi = "10.4238/2015.August.7.13",

language = "英语",

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AU - Liu, X.

AU - Yang, Y. Y.

AU - Wang, X. M.

AU - Liu, Z. S.

AU - Wang, Z. H.

AU - Ding, Y. Z.

N1 - Publisher Copyright: © FUNPEC-RP.

PY - 2015/8/7

Y1 - 2015/8/7

N2 - The use of noninvasive genetic sampling to identify the sex of wild animals is an extremely valuable and important tool in molecular ecology and wildlife conservation. Sex determination using the amelogenin gene has been conducted in many species because only a single pair of primers is required to amplify both X- and Y-linked alleles. However, this method has not been used in field research with the feces of wildlife. In this study, we applied this method to 222 fecal samples from wild blue sheep (Pseudois nayaur) using amelogenin primers (SE47/SE48) after testing the effectiveness of sex determination using tissue samples and fecal samples from blue sheep of known sex. We found this method to be highly reliable (80.2%) for blue sheep. Amelogenin can be used to identify the sex of wild animals using fecal samples.

AB - The use of noninvasive genetic sampling to identify the sex of wild animals is an extremely valuable and important tool in molecular ecology and wildlife conservation. Sex determination using the amelogenin gene has been conducted in many species because only a single pair of primers is required to amplify both X- and Y-linked alleles. However, this method has not been used in field research with the feces of wildlife. In this study, we applied this method to 222 fecal samples from wild blue sheep (Pseudois nayaur) using amelogenin primers (SE47/SE48) after testing the effectiveness of sex determination using tissue samples and fecal samples from blue sheep of known sex. We found this method to be highly reliable (80.2%) for blue sheep. Amelogenin can be used to identify the sex of wild animals using fecal samples.

KW - Amelogenin

KW - Blue sheep

KW - Fecal sample

KW - Non-invasive genetic sample

KW - Polymerase chain reaction-based sex-typing

UR - https://www.scopus.com/pages/publications/84938866272

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DO - 10.4238/2015.August.7.13

M3 - 文章

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AN - SCOPUS:84938866272

SN - 1676-5680

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SP - 9045

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JO - Genetics and Molecular Research

JF - Genetics and Molecular Research

IS - 3

ER -

Sex identification based on AMEL gene PCR amplification from blue sheep (Pseudois nayaur) fecal DNA samples

Abstract

Keywords

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