SERS nanoprobes for the monitoring of endogenous nitric oxide in living cells

Jing Cui; Kai Hu; Jia Jia Sun; Lu Lu Qu; Da Wei Li

doi:10.1016/j.bios.2016.04.094

SERS nanoprobes for the monitoring of endogenous nitric oxide in living cells

Jing Cui, Kai Hu, Jia Jia Sun, Lu Lu Qu, Da Wei Li

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

Nitric Oxide (NO) is a significant gaseous signalling molecule in various pathological and physiological pathways, whereas many of its functions are still ambiguous in part because of the shortage of powerful detection approaches. Herein, we present a type of reaction-based surface-enhanced Raman scattering (SERS) nanoprobes, o-phenylenediamine-modified gold nanoparticles (AuNPs/OPD), to detect the level of the endogenous NO in living cells. The detection is achieved through the SERS variation of AuNPs/OPD caused by the reaction between NO and OPD on the surface of AuNPs. The proposed SERS nanoprobes have a good stability and a rapid response to NO within 30 s Moreover, as a result of the reaction specificity coupled with SERS fingerprinting, AuNPs/OPD nanoprobes demonstrate high selectivity towards NO over other biologically relevant species with a sensitivity at 10^-7 M level. Thereby, this SERS strategy can be used for monitoring NO that is endogenously produced in living macrophages, indicating immense potential in studying NO-involved pathophysiological processes in biological systems.

Original language	English
Pages (from-to)	324-330
Number of pages	7
Journal	Biosensors and Bioelectronics
Volume	85
DOIs	https://doi.org/10.1016/j.bios.2016.04.094
State	Published - 15 Nov 2016
Externally published	Yes

Keywords

Living cells
Monitoring
Nitric oxide
SERS

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10.1016/j.bios.2016.04.094

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@article{cae192c0544b42dabafa869c54f5acd0,

title = "SERS nanoprobes for the monitoring of endogenous nitric oxide in living cells",

abstract = "Nitric Oxide (NO) is a significant gaseous signalling molecule in various pathological and physiological pathways, whereas many of its functions are still ambiguous in part because of the shortage of powerful detection approaches. Herein, we present a type of reaction-based surface-enhanced Raman scattering (SERS) nanoprobes, o-phenylenediamine-modified gold nanoparticles (AuNPs/OPD), to detect the level of the endogenous NO in living cells. The detection is achieved through the SERS variation of AuNPs/OPD caused by the reaction between NO and OPD on the surface of AuNPs. The proposed SERS nanoprobes have a good stability and a rapid response to NO within 30 s Moreover, as a result of the reaction specificity coupled with SERS fingerprinting, AuNPs/OPD nanoprobes demonstrate high selectivity towards NO over other biologically relevant species with a sensitivity at 10-7 M level. Thereby, this SERS strategy can be used for monitoring NO that is endogenously produced in living macrophages, indicating immense potential in studying NO-involved pathophysiological processes in biological systems.",

keywords = "Living cells, Monitoring, Nitric oxide, SERS",

author = "Jing Cui and Kai Hu and Sun, \{Jia Jia\} and Qu, \{Lu Lu\} and Li, \{Da Wei\}",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier B.V.",

year = "2016",

month = nov,

day = "15",

doi = "10.1016/j.bios.2016.04.094",

language = "英语",

volume = "85",

pages = "324--330",

journal = "Biosensors and Bioelectronics",

issn = "0956-5663",

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TY - JOUR

T1 - SERS nanoprobes for the monitoring of endogenous nitric oxide in living cells

AU - Cui, Jing

AU - Hu, Kai

AU - Sun, Jia Jia

AU - Qu, Lu Lu

AU - Li, Da Wei

PY - 2016/11/15

Y1 - 2016/11/15

N2 - Nitric Oxide (NO) is a significant gaseous signalling molecule in various pathological and physiological pathways, whereas many of its functions are still ambiguous in part because of the shortage of powerful detection approaches. Herein, we present a type of reaction-based surface-enhanced Raman scattering (SERS) nanoprobes, o-phenylenediamine-modified gold nanoparticles (AuNPs/OPD), to detect the level of the endogenous NO in living cells. The detection is achieved through the SERS variation of AuNPs/OPD caused by the reaction between NO and OPD on the surface of AuNPs. The proposed SERS nanoprobes have a good stability and a rapid response to NO within 30 s Moreover, as a result of the reaction specificity coupled with SERS fingerprinting, AuNPs/OPD nanoprobes demonstrate high selectivity towards NO over other biologically relevant species with a sensitivity at 10-7 M level. Thereby, this SERS strategy can be used for monitoring NO that is endogenously produced in living macrophages, indicating immense potential in studying NO-involved pathophysiological processes in biological systems.

AB - Nitric Oxide (NO) is a significant gaseous signalling molecule in various pathological and physiological pathways, whereas many of its functions are still ambiguous in part because of the shortage of powerful detection approaches. Herein, we present a type of reaction-based surface-enhanced Raman scattering (SERS) nanoprobes, o-phenylenediamine-modified gold nanoparticles (AuNPs/OPD), to detect the level of the endogenous NO in living cells. The detection is achieved through the SERS variation of AuNPs/OPD caused by the reaction between NO and OPD on the surface of AuNPs. The proposed SERS nanoprobes have a good stability and a rapid response to NO within 30 s Moreover, as a result of the reaction specificity coupled with SERS fingerprinting, AuNPs/OPD nanoprobes demonstrate high selectivity towards NO over other biologically relevant species with a sensitivity at 10-7 M level. Thereby, this SERS strategy can be used for monitoring NO that is endogenously produced in living macrophages, indicating immense potential in studying NO-involved pathophysiological processes in biological systems.

KW - Living cells

KW - Monitoring

KW - Nitric oxide

KW - SERS

UR - https://www.scopus.com/pages/publications/84966539679

U2 - 10.1016/j.bios.2016.04.094

DO - 10.1016/j.bios.2016.04.094

M3 - 文章

C2 - 27183283

AN - SCOPUS:84966539679

SN - 0956-5663

VL - 85

SP - 324

EP - 330

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

ER -

SERS nanoprobes for the monitoring of endogenous nitric oxide in living cells

Abstract

Keywords

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