TY - JOUR
T1 - Separation and determination of three water-soluble vitamins in pharmaceutical preparations and food by micellar electrokinetic chromatography with amperometric electrochemical detection
AU - Hu, Qin
AU - Zhou, Tianshu
AU - Zhang, Lan
AU - Li, Hong
AU - Fang, Yuzhi
PY - 2001/6/20
Y1 - 2001/6/20
N2 - A simple, reliable and reproducible method, based on micellar electrokinetic chromatography with amperometric detection, for the separation and determination of the three water-soluble vitamins, namely nicotinamide (VPP), pyridoxine (VB6) and ascorbic acid (VC), in pharmaceutical preparations and food is described in this paper. A carbon disk electrode was used as working electrode. The optimal conditions of separation and detection were 0.02 mol/l phosphate-0.005 mol/l borate buffer (pH 8.8) containing 0.03mol/l sodium dodecylsulphate (SDS), 7kV for the separation voltage, and 1.20V (versus Ag/AgCl) for the detection potential. The linear ranges were from 3.0 × 10-4 to 1.0 × 10-2 mol/l for VPP, 2.5 × 10-6 to 1.0 × 10-3 mol/l for VB6, and 2.5 × 10-6 to 1.0 × 10-3 mol/l for VC with regression coefficients of 0.9900, 0.9998 and 0.9990, respectively. The detection limits for VPP, VB6 and VC were 5.0 × 10-5, 1.0 × 10-6 and 1.0 × 10-6 mol/l, respectively. The method developed in this paper was directly applied to the determination of the three water-soluble vitamins in pharmaceutical preparations and food, and the assay results were satisfactory.
AB - A simple, reliable and reproducible method, based on micellar electrokinetic chromatography with amperometric detection, for the separation and determination of the three water-soluble vitamins, namely nicotinamide (VPP), pyridoxine (VB6) and ascorbic acid (VC), in pharmaceutical preparations and food is described in this paper. A carbon disk electrode was used as working electrode. The optimal conditions of separation and detection were 0.02 mol/l phosphate-0.005 mol/l borate buffer (pH 8.8) containing 0.03mol/l sodium dodecylsulphate (SDS), 7kV for the separation voltage, and 1.20V (versus Ag/AgCl) for the detection potential. The linear ranges were from 3.0 × 10-4 to 1.0 × 10-2 mol/l for VPP, 2.5 × 10-6 to 1.0 × 10-3 mol/l for VB6, and 2.5 × 10-6 to 1.0 × 10-3 mol/l for VC with regression coefficients of 0.9900, 0.9998 and 0.9990, respectively. The detection limits for VPP, VB6 and VC were 5.0 × 10-5, 1.0 × 10-6 and 1.0 × 10-6 mol/l, respectively. The method developed in this paper was directly applied to the determination of the three water-soluble vitamins in pharmaceutical preparations and food, and the assay results were satisfactory.
KW - Electrochemical detection
KW - Micellar electrokinetic chromatography
KW - Water-soluble vitamins
UR - https://www.scopus.com/pages/publications/0035919256
U2 - 10.1016/S0003-2670(01)00978-3
DO - 10.1016/S0003-2670(01)00978-3
M3 - 文章
AN - SCOPUS:0035919256
SN - 0003-2670
VL - 437
SP - 123
EP - 129
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 1
ER -