TY - JOUR
T1 - Sensitive analysis of aminoglycoside antibiotics via hyphenation of transient moving substitution boundary with field-enhanced sample injection in capillary electrophoresis
AU - Ge, Shuli
AU - Tang, Wanrong
AU - Han, Ruobing
AU - Zhu, Yan
AU - Wang, Qingjiang
AU - He, Pingang
AU - Fang, Yuzhi
PY - 2013/6/21
Y1 - 2013/6/21
N2 - A novel field-enhanced sample injection coupled with transient moving substitution boundary method in capillary electrophoresis was developed for aminoglycoside antibiotic (AG) analysis using 18-crown-6-tetracarboxylic acid (18C6H4) as a pseudostationary phase. Results indicated that the stacking mechanism of moving substitution boundary relied on the substitution reaction between 18C6H4-bonded AG complexes and Na+ at the substitution boundary. The stacking mechanism as well as important parameters governing pre-concentration and separation have been investigated in order to obtain maximum resolution and sensitivity. Under optimized conditions, using a sample prepared in a low-conductivity matrix, the limits of detection for streptomycin, neomycin, and kanamycin were 0.62, 5.9 and 8.6nM (S/N=3), respectively, and the detection sensitivities were improved 940-, 692-, and 415-fold, respectively. The method also gave accurate and reliable results in the analysis of AGs in river water samples.
AB - A novel field-enhanced sample injection coupled with transient moving substitution boundary method in capillary electrophoresis was developed for aminoglycoside antibiotic (AG) analysis using 18-crown-6-tetracarboxylic acid (18C6H4) as a pseudostationary phase. Results indicated that the stacking mechanism of moving substitution boundary relied on the substitution reaction between 18C6H4-bonded AG complexes and Na+ at the substitution boundary. The stacking mechanism as well as important parameters governing pre-concentration and separation have been investigated in order to obtain maximum resolution and sensitivity. Under optimized conditions, using a sample prepared in a low-conductivity matrix, the limits of detection for streptomycin, neomycin, and kanamycin were 0.62, 5.9 and 8.6nM (S/N=3), respectively, and the detection sensitivities were improved 940-, 692-, and 415-fold, respectively. The method also gave accurate and reliable results in the analysis of AGs in river water samples.
KW - 18C6H4
KW - Aminoglycoside antibiotics
KW - Amperometric detection
KW - Capillary zone electrophoresis
KW - Field-enhanced sample injection
KW - Moving substitution boundary
UR - https://www.scopus.com/pages/publications/84886777634
U2 - 10.1016/j.chroma.2013.04.049
DO - 10.1016/j.chroma.2013.04.049
M3 - 文章
C2 - 23669327
AN - SCOPUS:84886777634
SN - 0021-9673
VL - 1295
SP - 128
EP - 135
JO - Journal of Chromatography A
JF - Journal of Chromatography A
ER -