TY - JOUR
T1 - Selective and sensitive monitoring of cerebral antioxidants based on the dye-labeled DNA/polydopamine conjugates
AU - Ma, Shishi
AU - Qi, Yan Xia
AU - Jiang, Xiao Qin
AU - Chen, Jie Qiong
AU - Zhou, Qiao Yu
AU - Shi, Guoyue
AU - Zhang, Min
N1 - Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/12/6
Y1 - 2016/12/6
N2 - A simple and novel method for evaluating antioxidants in complex biological fluids has been developed based on the interaction of dye-labeled singlestrand DNA (ssDNA) and polydopamine (PDA). Due to the interaction between ssDNA and PDA, the fluorescence of dye-labeled ssDNA (e.g., FITC-ssDNA, as donor) can be quenched by PDA (as acceptor) to the fluorescence "off" state through Förster resonance energy transfer (FRET). However, in the presence of various antioxidants, such as glutathione (GSH), ascorbic acid (AA), cysteine (Cys), and homocysteine (Hcys), the spontaneous oxidative polymerization reaction from DA to PDA would be blocked, resulting in the freedom of FITC-ssDNA and leading to the fluorescence "on" state. The sensing system shows great sensitivity for the monitoring of antioxidants in a fluorescent "turn on" format. The new strategy also exhibits great selectivity and is free from the interferences of amino acids, metal ions and the biological species commonly existing in brain systems. Moreover, by combining the microdialysis technique, the present method has been successfully applied to monitor the dynamic changes of the striatum antioxidants in rat cerebrospinal microdialysates during the normal/ischemia/reperfusion process. This work establishes an effective platform for in vivo monitoring antioxidants in cerebral ischemia model, and promises new opportunities for the research of brain chemistry, neuroprotection, physiological, and pathological events.
AB - A simple and novel method for evaluating antioxidants in complex biological fluids has been developed based on the interaction of dye-labeled singlestrand DNA (ssDNA) and polydopamine (PDA). Due to the interaction between ssDNA and PDA, the fluorescence of dye-labeled ssDNA (e.g., FITC-ssDNA, as donor) can be quenched by PDA (as acceptor) to the fluorescence "off" state through Förster resonance energy transfer (FRET). However, in the presence of various antioxidants, such as glutathione (GSH), ascorbic acid (AA), cysteine (Cys), and homocysteine (Hcys), the spontaneous oxidative polymerization reaction from DA to PDA would be blocked, resulting in the freedom of FITC-ssDNA and leading to the fluorescence "on" state. The sensing system shows great sensitivity for the monitoring of antioxidants in a fluorescent "turn on" format. The new strategy also exhibits great selectivity and is free from the interferences of amino acids, metal ions and the biological species commonly existing in brain systems. Moreover, by combining the microdialysis technique, the present method has been successfully applied to monitor the dynamic changes of the striatum antioxidants in rat cerebrospinal microdialysates during the normal/ischemia/reperfusion process. This work establishes an effective platform for in vivo monitoring antioxidants in cerebral ischemia model, and promises new opportunities for the research of brain chemistry, neuroprotection, physiological, and pathological events.
UR - https://www.scopus.com/pages/publications/85019850427
U2 - 10.1021/acs.analchem.6b03216
DO - 10.1021/acs.analchem.6b03216
M3 - 文章
C2 - 27934126
AN - SCOPUS:85019850427
SN - 0003-2700
VL - 88
SP - 11647
EP - 11653
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 23
ER -