Screening and chromatographic assessing of a novel lgG biomimetic ligand

Hongqin Feng; Lingyun Jia; Honglin Li; Xicheng Wang

doi:10.1002/bmc.654

Screening and chromatographic assessing of a novel lgG biomimetic ligand

Hongqin Feng
, Lingyun Jia^*
, Honglin Li
, Xicheng Wang

^*Corresponding author for this work

Dalian University of Technology

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

A novel biormimetic ligand, N-benzyloxycarbonyl-L-tyrosine (N-cbZ-L-Tyr), was screened by a combination method of molecular docking and immobilized receptor technique. Then, N-cbZ-L-Tyr was immobilized on Sepharose CL-4B to prepare a specific affinity adsorbent for immunoglobulin G (IgG). Scatchard analysis of the binding isotherm for IgG on the adsorbent gave an association constant (K_a) of 4.91 × 10₆m_-1 and a theoretical maximum adsorption capacity of 17.3 mg IgG/mL gel. IgG with a purity of 98% was separated from human plasma by this new affinity adsorbent.

Original language	English
Pages (from-to)	1109-1115
Number of pages	7
Journal	Biomedical Chromatography
Volume	20
Issue number	10
DOIs	https://doi.org/10.1002/bmc.654
State	Published - Oct 2006
Externally published	Yes

Keywords

Affinity chromatography
Biomimetic ligand
IgG
Purification
Screening

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10.1002/bmc.654

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title = "Screening and chromatographic assessing of a novel lgG biomimetic ligand",

abstract = "A novel biormimetic ligand, N-benzyloxycarbonyl-L-tyrosine (N-cbZ-L-Tyr), was screened by a combination method of molecular docking and immobilized receptor technique. Then, N-cbZ-L-Tyr was immobilized on Sepharose CL-4B to prepare a specific affinity adsorbent for immunoglobulin G (IgG). Scatchard analysis of the binding isotherm for IgG on the adsorbent gave an association constant (Ka) of 4.91 × 106m-1 and a theoretical maximum adsorption capacity of 17.3 mg IgG/mL gel. IgG with a purity of 98\% was separated from human plasma by this new affinity adsorbent.",

keywords = "Affinity chromatography, Biomimetic ligand, IgG, Purification, Screening",

author = "Hongqin Feng and Lingyun Jia and Honglin Li and Xicheng Wang",

year = "2006",

month = oct,

doi = "10.1002/bmc.654",

language = "英语",

volume = "20",

pages = "1109--1115",

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TY - JOUR

T1 - Screening and chromatographic assessing of a novel lgG biomimetic ligand

AU - Feng, Hongqin

AU - Jia, Lingyun

AU - Li, Honglin

AU - Wang, Xicheng

PY - 2006/10

Y1 - 2006/10

N2 - A novel biormimetic ligand, N-benzyloxycarbonyl-L-tyrosine (N-cbZ-L-Tyr), was screened by a combination method of molecular docking and immobilized receptor technique. Then, N-cbZ-L-Tyr was immobilized on Sepharose CL-4B to prepare a specific affinity adsorbent for immunoglobulin G (IgG). Scatchard analysis of the binding isotherm for IgG on the adsorbent gave an association constant (Ka) of 4.91 × 106m-1 and a theoretical maximum adsorption capacity of 17.3 mg IgG/mL gel. IgG with a purity of 98% was separated from human plasma by this new affinity adsorbent.

AB - A novel biormimetic ligand, N-benzyloxycarbonyl-L-tyrosine (N-cbZ-L-Tyr), was screened by a combination method of molecular docking and immobilized receptor technique. Then, N-cbZ-L-Tyr was immobilized on Sepharose CL-4B to prepare a specific affinity adsorbent for immunoglobulin G (IgG). Scatchard analysis of the binding isotherm for IgG on the adsorbent gave an association constant (Ka) of 4.91 × 106m-1 and a theoretical maximum adsorption capacity of 17.3 mg IgG/mL gel. IgG with a purity of 98% was separated from human plasma by this new affinity adsorbent.

KW - Affinity chromatography

KW - Biomimetic ligand

KW - IgG

KW - Purification

KW - Screening

UR - https://www.scopus.com/pages/publications/33750684339

U2 - 10.1002/bmc.654

DO - 10.1002/bmc.654

M3 - 文章

C2 - 16708378

AN - SCOPUS:33750684339

SN - 0269-3879

VL - 20

SP - 1109

EP - 1115

JO - Biomedical Chromatography

JF - Biomedical Chromatography

IS - 10

ER -

Screening and chromatographic assessing of a novel lgG biomimetic ligand

Abstract

Keywords

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