Schiff Base Reaction in a Living Cell: In Situ Synthesis of a Hollow Covalent Organic Polymer To Regulate Biological Functions

Han Bin Xu; Hua Ying Chen; Jian Lv; Bin Bin Chen; Ze Rui Zhou; Shuai Chang; Ya Ting Gao; Wen Fei Huang; Ming Jie Ye; Zi Jian Cheng; Mahmoud Elsayed Hafez; Ruo Can Qian; Da Wei Li

doi:10.1002/anie.202311002

Schiff Base Reaction in a Living Cell: In Situ Synthesis of a Hollow Covalent Organic Polymer To Regulate Biological Functions

Han Bin Xu
, Hua Ying Chen
, Jian Lv
, Bin Bin Chen
, Ze Rui Zhou
, Shuai Chang
, Ya Ting Gao
, Wen Fei Huang
, Ming Jie Ye
, Zi Jian Cheng
, Mahmoud Elsayed Hafez
, Ruo Can Qian^*
, Da Wei Li^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Artificially performing chemical reactions in living biosystems to attain various physiological aims remains an intriguing but very challenging task. In this study, the Schiff base reaction was conducted in cells using Sc(OTf)₃ as a catalyst, enabling the in situ synthesis of a hollow covalent organic polymer (HCOP) without external stimuli. The reversible Schiff base reaction mediated intracellular Oswald ripening endows the HCOP with a spherical, hollow porous structure and a large specific surface area. The intracellularly generated HCOP reduced cellular motility by restraining actin polymerization, which consequently induced mitochondrial deactivation, apoptosis, and necroptosis. The presented intracellular synthesis system inspired by the Schiff base reaction has strong potential to regulate cell fate and biological functions, opening up a new strategic possibility for intervening in cellular behavior.

Original language	English
Article number	e202311002
Journal	Angewandte Chemie - International Edition
Volume	62
Issue number	44
DOIs	https://doi.org/10.1002/anie.202311002
State	Published - 26 Oct 2023
Externally published	Yes

Keywords

Biological Function Regulation
Hollow Covalent Organic Polymers
Intracellular Schiff Base Reaction
in Situ Biosynthesis

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10.1002/anie.202311002

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Xu, H. B., Chen, H. Y., Lv, J., Chen, B. B., Zhou, Z. R., Chang, S., Gao, Y. T., Huang, W. F., Ye, M. J., Cheng, Z. J., Hafez, M. E., Qian, R. C., & Li, D. W. (2023). Schiff Base Reaction in a Living Cell: In Situ Synthesis of a Hollow Covalent Organic Polymer To Regulate Biological Functions. Angewandte Chemie - International Edition, 62(44), Article e202311002. https://doi.org/10.1002/anie.202311002

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title = "Schiff Base Reaction in a Living Cell: In Situ Synthesis of a Hollow Covalent Organic Polymer To Regulate Biological Functions",

abstract = "Artificially performing chemical reactions in living biosystems to attain various physiological aims remains an intriguing but very challenging task. In this study, the Schiff base reaction was conducted in cells using Sc(OTf)3 as a catalyst, enabling the in situ synthesis of a hollow covalent organic polymer (HCOP) without external stimuli. The reversible Schiff base reaction mediated intracellular Oswald ripening endows the HCOP with a spherical, hollow porous structure and a large specific surface area. The intracellularly generated HCOP reduced cellular motility by restraining actin polymerization, which consequently induced mitochondrial deactivation, apoptosis, and necroptosis. The presented intracellular synthesis system inspired by the Schiff base reaction has strong potential to regulate cell fate and biological functions, opening up a new strategic possibility for intervening in cellular behavior.",

keywords = "Biological Function Regulation, Hollow Covalent Organic Polymers, Intracellular Schiff Base Reaction, in Situ Biosynthesis",

author = "Xu, \{Han Bin\} and Chen, \{Hua Ying\} and Jian Lv and Chen, \{Bin Bin\} and Zhou, \{Ze Rui\} and Shuai Chang and Gao, \{Ya Ting\} and Huang, \{Wen Fei\} and Ye, \{Ming Jie\} and Cheng, \{Zi Jian\} and Hafez, \{Mahmoud Elsayed\} and Qian, \{Ruo Can\} and Li, \{Da Wei\}",

note = "Publisher Copyright: {\textcopyright} 2023 Wiley-VCH GmbH.",

year = "2023",

month = oct,

day = "26",

doi = "10.1002/anie.202311002",

language = "英语",

volume = "62",

journal = "Angewandte Chemie - International Edition",

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TY - JOUR

T1 - Schiff Base Reaction in a Living Cell

T2 - In Situ Synthesis of a Hollow Covalent Organic Polymer To Regulate Biological Functions

AU - Xu, Han Bin

AU - Chen, Hua Ying

AU - Lv, Jian

AU - Chen, Bin Bin

AU - Zhou, Ze Rui

AU - Chang, Shuai

AU - Gao, Ya Ting

AU - Huang, Wen Fei

AU - Ye, Ming Jie

AU - Cheng, Zi Jian

AU - Hafez, Mahmoud Elsayed

AU - Qian, Ruo Can

AU - Li, Da Wei

PY - 2023/10/26

Y1 - 2023/10/26

N2 - Artificially performing chemical reactions in living biosystems to attain various physiological aims remains an intriguing but very challenging task. In this study, the Schiff base reaction was conducted in cells using Sc(OTf)3 as a catalyst, enabling the in situ synthesis of a hollow covalent organic polymer (HCOP) without external stimuli. The reversible Schiff base reaction mediated intracellular Oswald ripening endows the HCOP with a spherical, hollow porous structure and a large specific surface area. The intracellularly generated HCOP reduced cellular motility by restraining actin polymerization, which consequently induced mitochondrial deactivation, apoptosis, and necroptosis. The presented intracellular synthesis system inspired by the Schiff base reaction has strong potential to regulate cell fate and biological functions, opening up a new strategic possibility for intervening in cellular behavior.

AB - Artificially performing chemical reactions in living biosystems to attain various physiological aims remains an intriguing but very challenging task. In this study, the Schiff base reaction was conducted in cells using Sc(OTf)3 as a catalyst, enabling the in situ synthesis of a hollow covalent organic polymer (HCOP) without external stimuli. The reversible Schiff base reaction mediated intracellular Oswald ripening endows the HCOP with a spherical, hollow porous structure and a large specific surface area. The intracellularly generated HCOP reduced cellular motility by restraining actin polymerization, which consequently induced mitochondrial deactivation, apoptosis, and necroptosis. The presented intracellular synthesis system inspired by the Schiff base reaction has strong potential to regulate cell fate and biological functions, opening up a new strategic possibility for intervening in cellular behavior.

KW - Biological Function Regulation

KW - Hollow Covalent Organic Polymers

KW - Intracellular Schiff Base Reaction

KW - in Situ Biosynthesis

UR - https://www.scopus.com/pages/publications/85172694475

U2 - 10.1002/anie.202311002

DO - 10.1002/anie.202311002

M3 - 文章

C2 - 37714815

AN - SCOPUS:85172694475

SN - 1433-7851

VL - 62

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 44

M1 - e202311002

ER -

Schiff Base Reaction in a Living Cell: In Situ Synthesis of a Hollow Covalent Organic Polymer To Regulate Biological Functions

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Keywords

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