Abstract
To explore the role of doublesesx1 (dsx1) of Daphnia pulex in reproductive conversion, D. pulex of different reproductive stages were soaked in double-strand RNA (dsRNA) of dsx1 that had been synthesized in vitro. We adopted a dsx1 gene silencing method for D. pulex according to the characters of cuticula. Real-time PCR and whole-mount in situ hybridization were applied to detect the overall variation in dsx1 mRNA expression level in different individuals after interference. Real-time PCR result shows that dsx1 expression decreased significantly (P<0.05) in different reproductive stages in descending order of parthenogenetic females (68%), males (56%), and ephippial females (20%). Whole-mount in situ hybridization revealed that dsx1 expressed in the first antennae in males and ephippial females, whereas in the corresponding sites of parthenogenetic females, no expression was detected. Moreover, the expression was not detected in the first thoracic limb and compound eyes in all individuals, which is different from the situation before interference. Therefore, considering the expression patterns of different reproductive states, we believe that dsx1 is involved in the reproductive transformation and the maintenance of male sexual characteristics of D. pulex.
| Translated title of the contribution | Analysis of doublesex1 gene expression after RNA Interference in Daphnia pulex |
|---|---|
| Original language | Chinese (Traditional) |
| Pages (from-to) | 117-123 |
| Number of pages | 7 |
| Journal | Oceanologia et Limnologia Sinica |
| Volume | 49 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1 Jan 2018 |