Resolution of (D, L)-phenylalanine in biphasic system by -chymotrypsin immobilized on superparamagnetic nanogels

Jiahui Yu; Chengyun Wang; Jun Hong; Jin Huang

doi:10.1080/10601320903158263

Resolution of (D, L)-phenylalanine in biphasic system by -chymotrypsin immobilized on superparamagnetic nanogels

Jiahui Yu, Chengyun Wang, Jun Hong, Jin Huang

School of Chemistry and Molecular Engineering

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

-Chymotrypsin immobilized on super paramagnetic nanogels covered with carboxyl groups, exhibited high stereoselectivity in kinetic resolution of racemic phenylalanine in toluene/water biphasic system, and even improved reusability over its parent free enzyme. The results also showed that the addition of ionic liquid to water phase could enhance the stereoselectivity of the immobilized enzyme to a certain extent while the conversion yield decreased. The long-term stability allows the supported enzyme with nano-scaled dimension to use in continuous chiral resolution reaction, which opens a new horizon for enzymatic chiral resolution in large-scale production.

Original language	English
Pages (from-to)	943-948
Number of pages	6
Journal	Journal of Macromolecular Science, Part A: Pure and Applied Chemistry
Volume	46
Issue number	10
DOIs	https://doi.org/10.1080/10601320903158263
State	Published - Oct 2009

Keywords

(DL)-Phenylalanine
Enzymic resolution
Immobilized -chymotrypsinc
Ionic liquid
Superparamagnetic nanogels

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10.1080/10601320903158263

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title = "Resolution of (D, L)-phenylalanine in biphasic system by -chymotrypsin immobilized on superparamagnetic nanogels",

abstract = "-Chymotrypsin immobilized on super paramagnetic nanogels covered with carboxyl groups, exhibited high stereoselectivity in kinetic resolution of racemic phenylalanine in toluene/water biphasic system, and even improved reusability over its parent free enzyme. The results also showed that the addition of ionic liquid to water phase could enhance the stereoselectivity of the immobilized enzyme to a certain extent while the conversion yield decreased. The long-term stability allows the supported enzyme with nano-scaled dimension to use in continuous chiral resolution reaction, which opens a new horizon for enzymatic chiral resolution in large-scale production.",

keywords = "(DL)-Phenylalanine, Enzymic resolution, Immobilized -chymotrypsinc, Ionic liquid, Superparamagnetic nanogels",

author = "Jiahui Yu and Chengyun Wang and Jun Hong and Jin Huang",

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T1 - Resolution of (D, L)-phenylalanine in biphasic system by -chymotrypsin immobilized on superparamagnetic nanogels

AU - Yu, Jiahui

AU - Wang, Chengyun

AU - Hong, Jun

AU - Huang, Jin

PY - 2009/10

Y1 - 2009/10

N2 - -Chymotrypsin immobilized on super paramagnetic nanogels covered with carboxyl groups, exhibited high stereoselectivity in kinetic resolution of racemic phenylalanine in toluene/water biphasic system, and even improved reusability over its parent free enzyme. The results also showed that the addition of ionic liquid to water phase could enhance the stereoselectivity of the immobilized enzyme to a certain extent while the conversion yield decreased. The long-term stability allows the supported enzyme with nano-scaled dimension to use in continuous chiral resolution reaction, which opens a new horizon for enzymatic chiral resolution in large-scale production.

AB - -Chymotrypsin immobilized on super paramagnetic nanogels covered with carboxyl groups, exhibited high stereoselectivity in kinetic resolution of racemic phenylalanine in toluene/water biphasic system, and even improved reusability over its parent free enzyme. The results also showed that the addition of ionic liquid to water phase could enhance the stereoselectivity of the immobilized enzyme to a certain extent while the conversion yield decreased. The long-term stability allows the supported enzyme with nano-scaled dimension to use in continuous chiral resolution reaction, which opens a new horizon for enzymatic chiral resolution in large-scale production.

KW - (DL)-Phenylalanine

KW - Enzymic resolution

KW - Immobilized -chymotrypsinc

KW - Ionic liquid

KW - Superparamagnetic nanogels

UR - https://www.scopus.com/pages/publications/70349299185

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DO - 10.1080/10601320903158263

M3 - 文章

AN - SCOPUS:70349299185

SN - 1060-1325

VL - 46

SP - 943

EP - 948

JO - Journal of Macromolecular Science, Part A: Pure and Applied Chemistry

JF - Journal of Macromolecular Science, Part A: Pure and Applied Chemistry

IS - 10

ER -

Resolution of (D, L)-phenylalanine in biphasic system by -chymotrypsin immobilized on superparamagnetic nanogels

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