@article{f4977a97cf3b4d17b9c845e514b5f7fd,
title = "Rational Synthesis of Imine-Linked Fluorescent Covalent Organic Frameworks with Different p Kafor pH Sensing in Vitro and in Vivo",
abstract = "Precise modulation of pH in living cells plays a vital role in the study of many diseases, such as cancer and rheumatoid arthritis. Here, a series of imine-linked covalent organic frameworks (COFs) were rationally designed and developed for pH sensing in tumor cells and zebrafish. Four monomers were chosen to synthesize COFs (COF1-COF4) with different pKa by a simple orthogonal combination through condensation reaction. The as-obtained COFs exhibited a sensitive pH-dependent fluorescence response compared to their building blocks. Among them, COF2 possessed a high crystallinity, excellent fluorescence, and suitable pKa for biosensing. For bioimaging applications, COF2 was modified with poly-d-lysine (PDL) to improve its biocompatibility and endocytosis efficiency. After that, PDL-modified COF2 (PDL@COF2) was used as a novel fluorescence probe with a superior linear pH response over the range from 5.0 to 8.0 due to its fully reversible protonation and deprotonation. The fluorescent PDL@COF2 was further employed as a good candidate for pH imaging in tumor cells and zebrafish. The as-constructed environment-sensitive fluorescent COFs have greatly expanded the applications of COFs in the biological area.",
keywords = "cell imaging, covalent organic frameworks, pH detection, pH sensor, zebrafish imaging",
author = "Fang Yuan and Yujing Kong and Jia You and Cuiling Zhang and Yuezhong Xian",
note = "Publisher Copyright: {\textcopyright} 2021 American Chemical Society.",
year = "2021",
month = nov,
day = "3",
doi = "10.1021/acsami.1c14690",
language = "英语",
volume = "13",
pages = "51351--51361",
journal = "ACS Applied Materials and Interfaces",
issn = "1944-8244",
publisher = "American Chemical Society",
number = "43",
}