Rapid amperometric detection of coliforms based on MWNTs/Nafion composite film modified glass carbon electrode

Yuxiao Cheng; Yajun Liu; Jingjing Huang; Yuezhong Xian; Wen Zhang; Zhonghai Zhang; Litong Jin

doi:10.1016/j.talanta.2007.10.047

Rapid amperometric detection of coliforms based on MWNTs/Nafion composite film modified glass carbon electrode

Yuxiao Cheng, Yajun Liu, Jingjing Huang, Yuezhong Xian, Wen Zhang, Zhonghai Zhang, Litong Jin

School of Chemistry and Molecular Engineering

East China Normal University

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

A multi-wall carbon nanotubes (MWNTs)/Nafion modified glassy carbon electrode (GCE) was fabricated for the rapid amperometric detection of coliforms, represented by Escherichia coli (E. coli). In the bacterial solution, β-galactosidase which was used as an indicator of coliforms reacted with substrate, p-aminophenol-β-galactopyranoside (PAPG), and produced p-aminophenol (PAP). PAP was detected by MWNTs/Nafion modified GCE. Due to the cation-exchange capacity of Nafion and the electrocatalytic ability of MWNTs, the detection sensitivity of PAP was improved and the detection time of coliforms was shortened. The bacterial can be detected within 5 h ranging from 10 to 10⁴ cfu/mL. The MWNTs/Nafion modified GCE was easy to be constructed and regenerated. To our best knowledge, it was the first time to use MWNTs/Nafion modified GCE to detect the concentration of coliforms.

Original language	English
Pages (from-to)	167-171
Number of pages	5
Journal	Talanta
Volume	75
Issue number	1
DOIs	https://doi.org/10.1016/j.talanta.2007.10.047
State	Published - 15 Mar 2008

Keywords

Amperometric detection
Coliforms
Multi-wall carbon nanotubes
Nafion
p-Aminophenol
β-Galactosidase

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10.1016/j.talanta.2007.10.047

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title = "Rapid amperometric detection of coliforms based on MWNTs/Nafion composite film modified glass carbon electrode",

abstract = "A multi-wall carbon nanotubes (MWNTs)/Nafion modified glassy carbon electrode (GCE) was fabricated for the rapid amperometric detection of coliforms, represented by Escherichia coli (E. coli). In the bacterial solution, β-galactosidase which was used as an indicator of coliforms reacted with substrate, p-aminophenol-β-galactopyranoside (PAPG), and produced p-aminophenol (PAP). PAP was detected by MWNTs/Nafion modified GCE. Due to the cation-exchange capacity of Nafion and the electrocatalytic ability of MWNTs, the detection sensitivity of PAP was improved and the detection time of coliforms was shortened. The bacterial can be detected within 5 h ranging from 10 to 104 cfu/mL. The MWNTs/Nafion modified GCE was easy to be constructed and regenerated. To our best knowledge, it was the first time to use MWNTs/Nafion modified GCE to detect the concentration of coliforms.",

keywords = "Amperometric detection, Coliforms, Multi-wall carbon nanotubes, Nafion, p-Aminophenol, β-Galactosidase",

author = "Yuxiao Cheng and Yajun Liu and Jingjing Huang and Yuezhong Xian and Wen Zhang and Zhonghai Zhang and Litong Jin",

year = "2008",

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doi = "10.1016/j.talanta.2007.10.047",

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T1 - Rapid amperometric detection of coliforms based on MWNTs/Nafion composite film modified glass carbon electrode

AU - Cheng, Yuxiao

AU - Liu, Yajun

AU - Huang, Jingjing

AU - Xian, Yuezhong

AU - Zhang, Wen

AU - Zhang, Zhonghai

AU - Jin, Litong

PY - 2008/3/15

Y1 - 2008/3/15

N2 - A multi-wall carbon nanotubes (MWNTs)/Nafion modified glassy carbon electrode (GCE) was fabricated for the rapid amperometric detection of coliforms, represented by Escherichia coli (E. coli). In the bacterial solution, β-galactosidase which was used as an indicator of coliforms reacted with substrate, p-aminophenol-β-galactopyranoside (PAPG), and produced p-aminophenol (PAP). PAP was detected by MWNTs/Nafion modified GCE. Due to the cation-exchange capacity of Nafion and the electrocatalytic ability of MWNTs, the detection sensitivity of PAP was improved and the detection time of coliforms was shortened. The bacterial can be detected within 5 h ranging from 10 to 104 cfu/mL. The MWNTs/Nafion modified GCE was easy to be constructed and regenerated. To our best knowledge, it was the first time to use MWNTs/Nafion modified GCE to detect the concentration of coliforms.

AB - A multi-wall carbon nanotubes (MWNTs)/Nafion modified glassy carbon electrode (GCE) was fabricated for the rapid amperometric detection of coliforms, represented by Escherichia coli (E. coli). In the bacterial solution, β-galactosidase which was used as an indicator of coliforms reacted with substrate, p-aminophenol-β-galactopyranoside (PAPG), and produced p-aminophenol (PAP). PAP was detected by MWNTs/Nafion modified GCE. Due to the cation-exchange capacity of Nafion and the electrocatalytic ability of MWNTs, the detection sensitivity of PAP was improved and the detection time of coliforms was shortened. The bacterial can be detected within 5 h ranging from 10 to 104 cfu/mL. The MWNTs/Nafion modified GCE was easy to be constructed and regenerated. To our best knowledge, it was the first time to use MWNTs/Nafion modified GCE to detect the concentration of coliforms.

KW - Amperometric detection

KW - Coliforms

KW - Multi-wall carbon nanotubes

KW - Nafion

KW - p-Aminophenol

KW - β-Galactosidase

UR - https://www.scopus.com/pages/publications/39649103227

U2 - 10.1016/j.talanta.2007.10.047

DO - 10.1016/j.talanta.2007.10.047

M3 - 文章

AN - SCOPUS:39649103227

SN - 0039-9140

VL - 75

SP - 167

EP - 171

JO - Talanta

JF - Talanta

IS - 1

ER -

Rapid amperometric detection of coliforms based on MWNTs/Nafion composite film modified glass carbon electrode

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Keywords

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