Rac1-PAK2 pathway is essential for zebrafish heart regeneration

Xiangwen Peng; Quanze He; Guobao Li; Jinmin Ma; Tao P. Zhong

doi:10.1016/j.bbrc.2016.03.011

Rac1-PAK2 pathway is essential for zebrafish heart regeneration

Xiangwen Peng
, Quanze He
, Guobao Li
, Jinmin Ma
, Tao P. Zhong^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

P-21 activated kinases, or PAKs, are serine-threonine kinases that play important roles in diverse heart functions include heart development, cardiovascular development and function in a range of models; however, the mechanisms by which PAKs mediate heart regeneration are unknown. Here, we demonstrate that PAK2 and PAK4 expression is induced in cardiomyocytes and vessels, respectively, following zebrafish heart injury. Inhibition of PAK2 and PAK4 using a specific small molecule inhibitor impedes cardiomyocyte proliferation/dedifferentiation and cardiovascular regeneration, respectively. Cdc42 is specifically expressed in the ventricle and may function upstream of PAK2 but not PAK4 under normal conditions and that cardiomyocyte proliferentation during heart regeneration relies on Rac1-mediated activation of Pak2. Our results indicate that PAKs play a key role in heart regeneration.

Original language	English
Pages (from-to)	637-642
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	472
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2016.03.011
State	Published - 15 Apr 2016
Externally published	Yes

Keywords

Cardiovascular
Cdc42
Heart regeneration
PAK2
PAK4
Rac1
Zebrafish

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10.1016/j.bbrc.2016.03.011

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title = "Rac1-PAK2 pathway is essential for zebrafish heart regeneration",

abstract = "P-21 activated kinases, or PAKs, are serine-threonine kinases that play important roles in diverse heart functions include heart development, cardiovascular development and function in a range of models; however, the mechanisms by which PAKs mediate heart regeneration are unknown. Here, we demonstrate that PAK2 and PAK4 expression is induced in cardiomyocytes and vessels, respectively, following zebrafish heart injury. Inhibition of PAK2 and PAK4 using a specific small molecule inhibitor impedes cardiomyocyte proliferation/dedifferentiation and cardiovascular regeneration, respectively. Cdc42 is specifically expressed in the ventricle and may function upstream of PAK2 but not PAK4 under normal conditions and that cardiomyocyte proliferentation during heart regeneration relies on Rac1-mediated activation of Pak2. Our results indicate that PAKs play a key role in heart regeneration.",

keywords = "Cardiovascular, Cdc42, Heart regeneration, PAK2, PAK4, Rac1, Zebrafish",

author = "Xiangwen Peng and Quanze He and Guobao Li and Jinmin Ma and Zhong, \{Tao P.\}",

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doi = "10.1016/j.bbrc.2016.03.011",

language = "英语",

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TY - JOUR

T1 - Rac1-PAK2 pathway is essential for zebrafish heart regeneration

AU - Peng, Xiangwen

AU - He, Quanze

AU - Li, Guobao

AU - Ma, Jinmin

AU - Zhong, Tao P.

PY - 2016/4/15

Y1 - 2016/4/15

N2 - P-21 activated kinases, or PAKs, are serine-threonine kinases that play important roles in diverse heart functions include heart development, cardiovascular development and function in a range of models; however, the mechanisms by which PAKs mediate heart regeneration are unknown. Here, we demonstrate that PAK2 and PAK4 expression is induced in cardiomyocytes and vessels, respectively, following zebrafish heart injury. Inhibition of PAK2 and PAK4 using a specific small molecule inhibitor impedes cardiomyocyte proliferation/dedifferentiation and cardiovascular regeneration, respectively. Cdc42 is specifically expressed in the ventricle and may function upstream of PAK2 but not PAK4 under normal conditions and that cardiomyocyte proliferentation during heart regeneration relies on Rac1-mediated activation of Pak2. Our results indicate that PAKs play a key role in heart regeneration.

AB - P-21 activated kinases, or PAKs, are serine-threonine kinases that play important roles in diverse heart functions include heart development, cardiovascular development and function in a range of models; however, the mechanisms by which PAKs mediate heart regeneration are unknown. Here, we demonstrate that PAK2 and PAK4 expression is induced in cardiomyocytes and vessels, respectively, following zebrafish heart injury. Inhibition of PAK2 and PAK4 using a specific small molecule inhibitor impedes cardiomyocyte proliferation/dedifferentiation and cardiovascular regeneration, respectively. Cdc42 is specifically expressed in the ventricle and may function upstream of PAK2 but not PAK4 under normal conditions and that cardiomyocyte proliferentation during heart regeneration relies on Rac1-mediated activation of Pak2. Our results indicate that PAKs play a key role in heart regeneration.

KW - Cardiovascular

KW - Cdc42

KW - Heart regeneration

KW - PAK2

KW - PAK4

KW - Rac1

KW - Zebrafish

UR - https://www.scopus.com/pages/publications/84961221175

U2 - 10.1016/j.bbrc.2016.03.011

DO - 10.1016/j.bbrc.2016.03.011

M3 - 文章

C2 - 26966072

AN - SCOPUS:84961221175

SN - 0006-291X

VL - 472

SP - 637

EP - 642

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Rac1-PAK2 pathway is essential for zebrafish heart regeneration

Abstract

Keywords

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