Quenched fluorescein: A reference dye for instrument response function of TCSPC

Hai Feng Pan; Jing Xin Ding; Rong Rong Liang; Zhan Dong Tao; Meng Wei Liu; San Jun Zhang; Jian Hua Xu

doi:10.3964/j.issn.1000-0593(2014)08-2284-05

Quenched fluorescein: A reference dye for instrument response function of TCSPC

Hai Feng Pan, Jing Xin Ding, Rong Rong Liang, Zhan Dong Tao, Meng Wei Liu, San Jun Zhang^*, Jian Hua Xu

^*Corresponding author for this work

East China Normal University

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Measuring the instrument response function (IRF) and fitting by reconvolution algorithms are routines to improve time resolution in fluorescence lifetime measurements. Iodide ions were successfully used to quench the fluorescence of fluorescein in this study. By systematically adding saturated NaI water solution in basic fluorescein solution, the lifetimes of fluorescein were reduced from 4 ns to 24 ps. The quenched lifetime of fluorescein obtained from the analysis of Time-Correlated Single Photon Counting (TCSPC) measurement agrees well with that from femtosecond frequency up-conversion measurement. In time resolved excitation spectra measurements, the IRF should be measured at various detection wavelengths providing scattring materials are used. This study could not only reduce the complexity of IRF measurement, but also avoid the existing color effect in system. This study should have wide applications in time resolved fluorescence spectroscopy and fluorescence lifetime imaging.

Original language	English
Pages (from-to)	2284-2288
Number of pages	5
Journal	Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis
Volume	34
Issue number	8
DOIs	https://doi.org/10.3964/j.issn.1000-0593(2014)08-2284-05
State	Published - Aug 2014
Externally published	Yes

Keywords

Collision quenching
Fluorescein
Instrument response function
Time resolved fluorescence spectra

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10.3964/j.issn.1000-0593(2014)08-2284-05

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title = "Quenched fluorescein: A reference dye for instrument response function of TCSPC",

abstract = "Measuring the instrument response function (IRF) and fitting by reconvolution algorithms are routines to improve time resolution in fluorescence lifetime measurements. Iodide ions were successfully used to quench the fluorescence of fluorescein in this study. By systematically adding saturated NaI water solution in basic fluorescein solution, the lifetimes of fluorescein were reduced from 4 ns to 24 ps. The quenched lifetime of fluorescein obtained from the analysis of Time-Correlated Single Photon Counting (TCSPC) measurement agrees well with that from femtosecond frequency up-conversion measurement. In time resolved excitation spectra measurements, the IRF should be measured at various detection wavelengths providing scattring materials are used. This study could not only reduce the complexity of IRF measurement, but also avoid the existing color effect in system. This study should have wide applications in time resolved fluorescence spectroscopy and fluorescence lifetime imaging.",

keywords = "Collision quenching, Fluorescein, Instrument response function, Time resolved fluorescence spectra",

author = "Pan, \{Hai Feng\} and Ding, \{Jing Xin\} and Liang, \{Rong Rong\} and Tao, \{Zhan Dong\} and Liu, \{Meng Wei\} and Zhang, \{San Jun\} and Xu, \{Jian Hua\}",

year = "2014",

month = aug,

doi = "10.3964/j.issn.1000-0593(2014)08-2284-05",

language = "英语",

volume = "34",

pages = "2284--2288",

journal = "Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis",

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T1 - Quenched fluorescein

T2 - A reference dye for instrument response function of TCSPC

AU - Pan, Hai Feng

AU - Ding, Jing Xin

AU - Liang, Rong Rong

AU - Tao, Zhan Dong

AU - Liu, Meng Wei

AU - Zhang, San Jun

AU - Xu, Jian Hua

PY - 2014/8

Y1 - 2014/8

N2 - Measuring the instrument response function (IRF) and fitting by reconvolution algorithms are routines to improve time resolution in fluorescence lifetime measurements. Iodide ions were successfully used to quench the fluorescence of fluorescein in this study. By systematically adding saturated NaI water solution in basic fluorescein solution, the lifetimes of fluorescein were reduced from 4 ns to 24 ps. The quenched lifetime of fluorescein obtained from the analysis of Time-Correlated Single Photon Counting (TCSPC) measurement agrees well with that from femtosecond frequency up-conversion measurement. In time resolved excitation spectra measurements, the IRF should be measured at various detection wavelengths providing scattring materials are used. This study could not only reduce the complexity of IRF measurement, but also avoid the existing color effect in system. This study should have wide applications in time resolved fluorescence spectroscopy and fluorescence lifetime imaging.

AB - Measuring the instrument response function (IRF) and fitting by reconvolution algorithms are routines to improve time resolution in fluorescence lifetime measurements. Iodide ions were successfully used to quench the fluorescence of fluorescein in this study. By systematically adding saturated NaI water solution in basic fluorescein solution, the lifetimes of fluorescein were reduced from 4 ns to 24 ps. The quenched lifetime of fluorescein obtained from the analysis of Time-Correlated Single Photon Counting (TCSPC) measurement agrees well with that from femtosecond frequency up-conversion measurement. In time resolved excitation spectra measurements, the IRF should be measured at various detection wavelengths providing scattring materials are used. This study could not only reduce the complexity of IRF measurement, but also avoid the existing color effect in system. This study should have wide applications in time resolved fluorescence spectroscopy and fluorescence lifetime imaging.

KW - Collision quenching

KW - Fluorescein

KW - Instrument response function

KW - Time resolved fluorescence spectra

UR - https://www.scopus.com/pages/publications/84905401760

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DO - 10.3964/j.issn.1000-0593(2014)08-2284-05

M3 - 文章

AN - SCOPUS:84905401760

SN - 1000-0593

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SP - 2284

EP - 2288

JO - Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis

JF - Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis

IS - 8

ER -

Quenched fluorescein: A reference dye for instrument response function of TCSPC

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Keywords

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