Purification of vitellin from the ovary of Chinese mitten-handed crab (Eriocheir sinensis) and development of an antivitellin ELISA

Liqiao Chen; Hongbo Jiang; Zhongliang Zhou; Kang Li; Kai Li; Glenn Y. Deng; Zhanjiang Liu

doi:10.1016/j.cbpc.2004.04.012

Purification of vitellin from the ovary of Chinese mitten-handed crab (Eriocheir sinensis) and development of an antivitellin ELISA

Liqiao Chen^*
, Hongbo Jiang
, Zhongliang Zhou
, Kang Li
, Kai Li
, Glenn Y. Deng
, Zhanjiang Liu

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Vitellin was purified from ovaries of mature female Chinese mitten-handed crab (Eriocheir sinensis) using gel filtration chromatography. Analysis by native PAGE showed the vitellin had a native molecular mass of 520 kDa, while denaturing SDS-PAGE revealed two subunits of 97 and 74 kDa. Purified vitellin was used to raise polyclonal antisera, with which an enzyme-linked immunosorbent assay (ELISA) was developed. The ELISA was sensitive and could effectively detect vitellin in the range of 7.8-500 ng. Furthermore, vitellin levels in various developmental stages of oogenesis were measured with the ELISA assay. The results indicated that levels of vitellin increased significantly from 0.22 mg/ovary at Stage II to 360.31 mg/ovary at Stage IV.

Original language	English
Pages (from-to)	305-311
Number of pages	7
Journal	Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
Volume	138
Issue number	3
DOIs	https://doi.org/10.1016/j.cbpc.2004.04.012
State	Published - Jul 2004

Keywords

Decapoda
Developmental stages
Enzyme-linked immunosorbent assay
Eriocheir sinensis
Gel filtration chromatography
Ovary
Purification
Vitellin

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10.1016/j.cbpc.2004.04.012

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@article{758ac474aec849c2bfcb18360a065d5e,

title = "Purification of vitellin from the ovary of Chinese mitten-handed crab (Eriocheir sinensis) and development of an antivitellin ELISA",

abstract = "Vitellin was purified from ovaries of mature female Chinese mitten-handed crab (Eriocheir sinensis) using gel filtration chromatography. Analysis by native PAGE showed the vitellin had a native molecular mass of 520 kDa, while denaturing SDS-PAGE revealed two subunits of 97 and 74 kDa. Purified vitellin was used to raise polyclonal antisera, with which an enzyme-linked immunosorbent assay (ELISA) was developed. The ELISA was sensitive and could effectively detect vitellin in the range of 7.8-500 ng. Furthermore, vitellin levels in various developmental stages of oogenesis were measured with the ELISA assay. The results indicated that levels of vitellin increased significantly from 0.22 mg/ovary at Stage II to 360.31 mg/ovary at Stage IV.",

keywords = "Decapoda, Developmental stages, Enzyme-linked immunosorbent assay, Eriocheir sinensis, Gel filtration chromatography, Ovary, Purification, Vitellin",

author = "Liqiao Chen and Hongbo Jiang and Zhongliang Zhou and Kang Li and Kai Li and Deng, \{Glenn Y.\} and Zhanjiang Liu",

year = "2004",

month = jul,

doi = "10.1016/j.cbpc.2004.04.012",

language = "英语",

volume = "138",

pages = "305--311",

journal = "Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology",

issn = "1096-4959",

publisher = "Elsevier Inc.",

number = "3",

}

Purification of vitellin from the ovary of Chinese mitten-handed crab (Eriocheir sinensis) and development of an antivitellin ELISA. / Chen, Liqiao; Jiang, Hongbo; Zhou, Zhongliang et al.
In: Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology, Vol. 138, No. 3, 07.2004, p. 305-311.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Purification of vitellin from the ovary of Chinese mitten-handed crab (Eriocheir sinensis) and development of an antivitellin ELISA

AU - Chen, Liqiao

AU - Jiang, Hongbo

AU - Zhou, Zhongliang

AU - Li, Kang

AU - Li, Kai

AU - Deng, Glenn Y.

AU - Liu, Zhanjiang

PY - 2004/7

Y1 - 2004/7

N2 - Vitellin was purified from ovaries of mature female Chinese mitten-handed crab (Eriocheir sinensis) using gel filtration chromatography. Analysis by native PAGE showed the vitellin had a native molecular mass of 520 kDa, while denaturing SDS-PAGE revealed two subunits of 97 and 74 kDa. Purified vitellin was used to raise polyclonal antisera, with which an enzyme-linked immunosorbent assay (ELISA) was developed. The ELISA was sensitive and could effectively detect vitellin in the range of 7.8-500 ng. Furthermore, vitellin levels in various developmental stages of oogenesis were measured with the ELISA assay. The results indicated that levels of vitellin increased significantly from 0.22 mg/ovary at Stage II to 360.31 mg/ovary at Stage IV.

AB - Vitellin was purified from ovaries of mature female Chinese mitten-handed crab (Eriocheir sinensis) using gel filtration chromatography. Analysis by native PAGE showed the vitellin had a native molecular mass of 520 kDa, while denaturing SDS-PAGE revealed two subunits of 97 and 74 kDa. Purified vitellin was used to raise polyclonal antisera, with which an enzyme-linked immunosorbent assay (ELISA) was developed. The ELISA was sensitive and could effectively detect vitellin in the range of 7.8-500 ng. Furthermore, vitellin levels in various developmental stages of oogenesis were measured with the ELISA assay. The results indicated that levels of vitellin increased significantly from 0.22 mg/ovary at Stage II to 360.31 mg/ovary at Stage IV.

KW - Decapoda

KW - Developmental stages

KW - Enzyme-linked immunosorbent assay

KW - Eriocheir sinensis

KW - Gel filtration chromatography

KW - Ovary

KW - Purification

KW - Vitellin

UR - https://www.scopus.com/pages/publications/3142564403

U2 - 10.1016/j.cbpc.2004.04.012

DO - 10.1016/j.cbpc.2004.04.012

M3 - 文章

C2 - 15253879

AN - SCOPUS:3142564403

SN - 1096-4959

VL - 138

SP - 305

EP - 311

JO - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology

IS - 3

ER -

Purification of vitellin from the ovary of Chinese mitten-handed crab (Eriocheir sinensis) and development of an antivitellin ELISA

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Keywords

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