Purification of allophycocyanin from Porphyra haitanensis and prepraration of its polyclonal antibody

Bowen Li; Zhenghong Zuo; Hongpo Dong; Yongbin Li; Yixin Chen

Purification of allophycocyanin from Porphyra haitanensis and prepraration of its polyclonal antibody

Bowen Li^*, Zhenghong Zuo, Hongpo Dong, Yongbin Li, Yixin Chen

^*Corresponding author for this work

Xiamen University

Research output: Contribution to journal › Article › peer-review

Abstract

In this study, a series of methods including grinding in the presence of liquid N₂, ultrasonic disruption, ammonium sulfate precipitation and gel filtration chromatography on DEAE-Sephadex-A-50 were applied to isolation of allophycocyanin, a crucial chlorophyll-protein in the photosynthesis of algae and an important marine bio-active substance, from Porphyra haitanensis. The purified allophycocyanin was injected into rabbits to prepare its polyclonal antibody, and specificity of polyclonal antibody was detected by Western blotting. The results indicated that the purified allophycocyanin producted two bands on SDS-PAGE, which denoted the a and β subunit of the allophycocyanin, and the purity and rate of recovery of allophycocyanin obtained were high, up to 3.5 (A₆₅₀/A₂₈₀) and 70.5% respectively. The result of indirect ELISA assay showed that the liter of the polyclonal antibody was nearly 1: 100000.

Original language	English
Pages (from-to)	638-642
Number of pages	5
Journal	Gaojishu Tongxin/Chinese High Technology Letters
Volume	16
Issue number	6
State	Published - Jun 2006
Externally published	Yes

Keywords

Allophycocyanin
Polyclonal-antibody
Porphyra haitanensis
Purification

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Purification of allophycocyanin from Porphyra haitanensis and prepraration of its polyclonal antibody",

abstract = "In this study, a series of methods including grinding in the presence of liquid N2, ultrasonic disruption, ammonium sulfate precipitation and gel filtration chromatography on DEAE-Sephadex-A-50 were applied to isolation of allophycocyanin, a crucial chlorophyll-protein in the photosynthesis of algae and an important marine bio-active substance, from Porphyra haitanensis. The purified allophycocyanin was injected into rabbits to prepare its polyclonal antibody, and specificity of polyclonal antibody was detected by Western blotting. The results indicated that the purified allophycocyanin producted two bands on SDS-PAGE, which denoted the a and β subunit of the allophycocyanin, and the purity and rate of recovery of allophycocyanin obtained were high, up to 3.5 (A650/A280) and 70.5\% respectively. The result of indirect ELISA assay showed that the liter of the polyclonal antibody was nearly 1: 100000.",

keywords = "Allophycocyanin, Polyclonal-antibody, Porphyra haitanensis, Purification",

author = "Bowen Li and Zhenghong Zuo and Hongpo Dong and Yongbin Li and Yixin Chen",

year = "2006",

month = jun,

language = "英语",

volume = "16",

pages = "638--642",

journal = "Gaojishu Tongxin/Chinese High Technology Letters",

issn = "1002-0470",

publisher = "Institute of Scientific and Technical Information of China ",

number = "6",

}

TY - JOUR

T1 - Purification of allophycocyanin from Porphyra haitanensis and prepraration of its polyclonal antibody

AU - Li, Bowen

AU - Zuo, Zhenghong

AU - Dong, Hongpo

AU - Li, Yongbin

AU - Chen, Yixin

PY - 2006/6

Y1 - 2006/6

N2 - In this study, a series of methods including grinding in the presence of liquid N2, ultrasonic disruption, ammonium sulfate precipitation and gel filtration chromatography on DEAE-Sephadex-A-50 were applied to isolation of allophycocyanin, a crucial chlorophyll-protein in the photosynthesis of algae and an important marine bio-active substance, from Porphyra haitanensis. The purified allophycocyanin was injected into rabbits to prepare its polyclonal antibody, and specificity of polyclonal antibody was detected by Western blotting. The results indicated that the purified allophycocyanin producted two bands on SDS-PAGE, which denoted the a and β subunit of the allophycocyanin, and the purity and rate of recovery of allophycocyanin obtained were high, up to 3.5 (A650/A280) and 70.5% respectively. The result of indirect ELISA assay showed that the liter of the polyclonal antibody was nearly 1: 100000.

AB - In this study, a series of methods including grinding in the presence of liquid N2, ultrasonic disruption, ammonium sulfate precipitation and gel filtration chromatography on DEAE-Sephadex-A-50 were applied to isolation of allophycocyanin, a crucial chlorophyll-protein in the photosynthesis of algae and an important marine bio-active substance, from Porphyra haitanensis. The purified allophycocyanin was injected into rabbits to prepare its polyclonal antibody, and specificity of polyclonal antibody was detected by Western blotting. The results indicated that the purified allophycocyanin producted two bands on SDS-PAGE, which denoted the a and β subunit of the allophycocyanin, and the purity and rate of recovery of allophycocyanin obtained were high, up to 3.5 (A650/A280) and 70.5% respectively. The result of indirect ELISA assay showed that the liter of the polyclonal antibody was nearly 1: 100000.

KW - Allophycocyanin

KW - Polyclonal-antibody

KW - Porphyra haitanensis

KW - Purification

UR - https://www.scopus.com/pages/publications/33745749370

M3 - 文章

AN - SCOPUS:33745749370

SN - 1002-0470

VL - 16

SP - 638

EP - 642

JO - Gaojishu Tongxin/Chinese High Technology Letters

JF - Gaojishu Tongxin/Chinese High Technology Letters

IS - 6

ER -

Purification of allophycocyanin from Porphyra haitanensis and prepraration of its polyclonal antibody

Abstract

Keywords

UN SDGs

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