Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304

Pazilat Bahti; Shunmei Chen; Yang Li; Neil Shaw; Xuejun Zhang; Min Zhang; Chongyun Cheng; Gaojie Song; Jie Yin; Hua Zhang; Dongsheng Che; Abdulla Abbas; Hao Xu; Bi Cheng Wang; Zhi Jie Liu

doi:10.1107/S1744309107068649

Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304

Pazilat Bahti
, Shunmei Chen
, Yang Li
, Neil Shaw
, Xuejun Zhang
, Min Zhang
, Chongyun Cheng
, Gaojie Song
, Jie Yin
, Hua Zhang
, Dongsheng Che
, Abdulla Abbas
, Hao Xu
, Bi Cheng Wang
, Zhi Jie Liu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

A 10.5 kDa non-Pfam hypothetical protein, AF1514, from the hyperthermophilic archaeon Archeoglobus fulgidus has been overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted X-rays to 2.09 Å resolution and a data set was collected at 100 K using Cu Kα radiation from a rotating-anode X-ray source. The crystals belong to space group P4 ₁2₁2 or P4₃2₁2, with unit-cell parameters a = b = 49.27, c = 106.61 Å. The calculated Matthews coefficient was 3.16 Å³ Da^-1, suggesting the presence of one molecule in the asymmetric unit.

Original language	English
Pages (from-to)	91-93
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	64
Issue number	2
DOIs	https://doi.org/10.1107/S1744309107068649
State	Published - 2008
Externally published	Yes

Keywords

Non-Pfam proteins
Reductive methylation

Access to Document

10.1107/S1744309107068649

Cite this

Bahti, P., Chen, S., Li, Y., Shaw, N., Zhang, X., Zhang, M., Cheng, C., Song, G., Yin, J., Zhang, H., Che, D., Abbas, A., Xu, H., Wang, B. C., & Liu, Z. J. (2008). Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 64(2), 91-93. https://doi.org/10.1107/S1744309107068649

@article{dcacc9c844964bc3bed43a138d2efebb,

title = "Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304",

abstract = "A 10.5 kDa non-Pfam hypothetical protein, AF1514, from the hyperthermophilic archaeon Archeoglobus fulgidus has been overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted X-rays to 2.09 {\AA} resolution and a data set was collected at 100 K using Cu Kα radiation from a rotating-anode X-ray source. The crystals belong to space group P4 1212 or P43212, with unit-cell parameters a = b = 49.27, c = 106.61 {\AA}. The calculated Matthews coefficient was 3.16 {\AA}3 Da-1, suggesting the presence of one molecule in the asymmetric unit.",

keywords = "Non-Pfam proteins, Reductive methylation",

author = "Pazilat Bahti and Shunmei Chen and Yang Li and Neil Shaw and Xuejun Zhang and Min Zhang and Chongyun Cheng and Gaojie Song and Jie Yin and Hua Zhang and Dongsheng Che and Abdulla Abbas and Hao Xu and Wang, \{Bi Cheng\} and Liu, \{Zhi Jie\}",

year = "2008",

doi = "10.1107/S1744309107068649",

language = "英语",

volume = "64",

pages = "91--93",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

issn = "1744-3091",

publisher = "John Wiley and Sons Ltd",

number = "2",

}

Bahti, P, Chen, S, Li, Y, Shaw, N, Zhang, X, Zhang, M, Cheng, C, Song, G, Yin, J, Zhang, H, Che, D, Abbas, A, Xu, H, Wang, BC & Liu, ZJ 2008, 'Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 64, no. 2, pp. 91-93. https://doi.org/10.1107/S1744309107068649

Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304. / Bahti, Pazilat; Chen, Shunmei; Li, Yang et al.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 64, No. 2, 2008, p. 91-93.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304

AU - Bahti, Pazilat

AU - Chen, Shunmei

AU - Li, Yang

AU - Shaw, Neil

AU - Zhang, Xuejun

AU - Zhang, Min

AU - Cheng, Chongyun

AU - Song, Gaojie

AU - Yin, Jie

AU - Zhang, Hua

AU - Che, Dongsheng

AU - Abbas, Abdulla

AU - Xu, Hao

AU - Wang, Bi Cheng

AU - Liu, Zhi Jie

PY - 2008

Y1 - 2008

N2 - A 10.5 kDa non-Pfam hypothetical protein, AF1514, from the hyperthermophilic archaeon Archeoglobus fulgidus has been overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted X-rays to 2.09 Å resolution and a data set was collected at 100 K using Cu Kα radiation from a rotating-anode X-ray source. The crystals belong to space group P4 1212 or P43212, with unit-cell parameters a = b = 49.27, c = 106.61 Å. The calculated Matthews coefficient was 3.16 Å3 Da-1, suggesting the presence of one molecule in the asymmetric unit.

AB - A 10.5 kDa non-Pfam hypothetical protein, AF1514, from the hyperthermophilic archaeon Archeoglobus fulgidus has been overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted X-rays to 2.09 Å resolution and a data set was collected at 100 K using Cu Kα radiation from a rotating-anode X-ray source. The crystals belong to space group P4 1212 or P43212, with unit-cell parameters a = b = 49.27, c = 106.61 Å. The calculated Matthews coefficient was 3.16 Å3 Da-1, suggesting the presence of one molecule in the asymmetric unit.

KW - Non-Pfam proteins

KW - Reductive methylation

UR - https://www.scopus.com/pages/publications/40449122524

U2 - 10.1107/S1744309107068649

DO - 10.1107/S1744309107068649

M3 - 文章

C2 - 18259057

AN - SCOPUS:40449122524

SN - 1744-3091

VL - 64

SP - 91

EP - 93

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

IS - 2

ER -

Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this